一测多评法同时测定活血丸中6个成分

Simultaneous determination of six constituents in Huoxue pills by QAMS

目的:建立一测多评法同时测定活血丸(当归、红花、大黄、猪牙皂、牵牛子)中没食子酸、儿茶素、阿魏酸、大黄酸、大黄素、大黄酚6个成分的含量。方法:该药物甲醇提取液的分析采用Agilent ZORBAX SB-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相甲醇-0.1%磷酸水溶液,梯度洗脱;体积流量1.0 mL·min^(-1);柱温30℃;检测波长为225、245、278、320 nm。以大黄酸为内标,计算其他5个成分相对校正因子,测定其含量。结果:6个成分在各自浓度范围内呈良好的线性关系(r≥0.9997),平均加样回收率为97.2%~100.8%(RSD为1.6%~2.0%)。一测多评法所得6个成分含量数据与外标法接近。结论:该方法简便、快捷,且稳定性好,准确度高,可为活血丸新药研发的质量控制研究提供依据。

Objective:To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of gallic acid,catechin,ferulic acid,rhein,emodin and chrysophanol in Huoxue pills(angelica sinensis,safflower,rhubarb,fructus gleditsiae and morning glory).Methods:The analysis of methanol extract of this drug was performed on a thermostatic Agilent ZORBAX SB-C_(18) column(250 mm×4.6 mm,5μm)at 30℃ with the mobile phase comprising of methanol-0.1% phosphoric acid aqueous solution at 1.0 mL·min^(-1) in a gradient elution manner,and the detection wavelengths were set at 225 nm,245 nm,278 nm and 320 nm.Rhein was used as an internal standard to calculate the relative correction factors of the other seven constituents,after which the content determination was made.Results:Six constituents showed good linear relationships within their own ranges(r≥0.9997),whose average recoveries were 97.2%-100.8% with RSDs of 1.6%-2.0%.The results obtained by QAMS approximated those obtained by external standard method.Conclusion:The method is simple,rapid,stable and accurate,and it can provide the basis for the quality control of Huoxue pills.