摘要
香稻拥有浓郁的香味和较高的营养价值,深得消费者的喜爱和育种专家的重视,挖掘和利用香稻基因资源为培育优质香稻品种奠定基础。水稻甜菜碱醛脱氢酶基因(OsBADH2)功能的丧失导致稻米具有香味。通过对10份香稻品种OsBADH2编码区进行测序分析,明确其变异类型。根据香稻OsBADH2第7外显子8 bp的缺失,设计功能标记E7,利用E7分别对测序的10份香稻品种和2个香稻/非香稻育种群体的OsBADH2基因型进行验证与检测。结果表明,象牙香占、花香B、农香24等9份香稻均在第7外显子发生8 bp的缺失和3 bp的替换,而香稻Xiao7在第14外显子有1个碱基(G)的插入突变。功能标记E7能快速准确地鉴定OsBADH2的纯合显性、纯合隐性和杂合基因型。
Fragrant rice is favored worldwide by consumers and rice breeders,due to its full aroma and high nutritional value.Exploring and utilizing the flagrant gene resources would lay a foundation for breeding excellent-quality varieties.Loss of function of the betaine aldehyde dehydrogenase gene(OsBADH2)leads to that rice is of fragrance.OsBADH2 coding regions in 10 fragrant rice varieties were sequenced to explicit its mutant type.A functional marker E7 was designed according to the 8-bp deletion in exon 7 of OsBADH2 in the fragrant rice.Genotypes of 10 fragrant rice varieties and 2 populations derived from fragrant rice/non-fragrant varieties were identified and verified by E7.The results indicated that 8 bp deletion and 3 bp replacement in the exon 7 of OsBADH2 gene were found in 9 cultivars,including Xiangyaxiangzhan,Huaxiang B and Nongxiang 24 et a1.And 1 bp(G)insertion mutation in exon 14 in the fragrant rice of Xiao7 appeared.The homozygous dominant,homozygous recessive and heterozygous genotypes of OsBADH2 could be rapidly and accurately distinguished by functional marker E7.
作者
孙平勇
张武汉
舒服
何强
张莉
彭志荣
邓华凤
SUN Ping-yong;ZHANG Wu-han;SHU Fu;HE Qiang;ZHANG Li;PENG Zhi-rong;DENG Hua-feng(State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125;Nuclear Agriculture and Space Breeding Research Institute,Changsha 410125;Hunan Academy of Agricultural Sciences,Changsha 410125)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第4期1-7,共7页
Biotechnology Bulletin
基金
长沙市科技计划项目(kq2004101)
湖南省重大专项(2018NK1020-1)
湖南农业科技创新资金(2020CX08,2019TD06)。