转录组测序分析毒死蜱与醚菊酯混配对二化螟毒杀的增效机制

Study on the synergistic mechanism of chlorpyrifos and ethofenprox mixture in poisoning Chilo suppressalis based on transcriptome sequencing

为明确毒死蜱与醚菊酯混配对二化螟毒杀的增效机制,采用联合毒力指数法筛选毒死蜱与醚菊酯混配的增效比例,利用转录组测序分析毒死蜱与醚菊酯混配的增效机制。结果表明,毒死蜱与醚菊酯以1∶2(质量比)混配时表现出增效作用,Illumina HiSeqTM X Ten测序获得高质量的二化螟转录组。去离子水处理与毒死蜱处理、去离子水处理与醚菊酯处理、去离子水处理与增效混配剂处理、毒死蜱处理与增效混配剂处理、醚菊酯处理与增效混配剂处理的差异表达基因分别为465、392、1874、117、25个。对毒死蜱与醚菊酯混配增效主要涉及的代谢过程进行通路分析,结合维恩交集法进行分析,发现解毒代谢酶可能介导毒死蜱与醚菊酯混配的增效机制。实时荧光定量PCR结果证实差异基因的表达量与测序数据匹配。本研究结果可为解析杀虫剂混配增效机制提供分子基础,为优化杀虫剂混配比例,建立二化螟抗性治理策略提供理论依据。

To reveal the synergistic mechanism of chlorpyrifos and ethofenprox mixture in poisoning Chilo suppressalis,the synergistic ratios of chlorpyrifos and ethofenprox mixture were screened by combined toxicity index method,the synergistic mechanism of chlorpyrifos and ethofenprox mixture were analyzed by transcriptome sequencing.The results indicated that chlorpyrifos and ethofenprox mixed at a 1∶2 mass ratio showed significant synergism on C.suppressalis.HiSeqTM X Ten platform was used to obtain C.suppressalis transcriptome with high quality data.465,392,1874,117 and 25 differentially expressed genes were obtained between the groups of deionized water and chlorpyrifos,deionized water and ethofenprox,deionized water and synergistic mixture,chlorpyrifos and synergistic mixture,ethofenprox and synergistic mixture.Pathway analysis showed that synergistic effect of chlorpyrifos and ethofenprox mixture was mainly involved in the metabolic process,and it was speculated that detoxification metabolic enzymes might mediate synergism mechanism of chlorpyrifos and ethofenprox combined with Venn intersection algorithm.The expression of significant different genes was highly matched with transcriptome sequencing data by quantitative real-time polymerase chain reaction(PCR).The results can provide a molecular basis for analyzing synergism mechanism of insecticide mixtures,and provide a theoretical basis for optimizing ratio of insecticide mixtures and establishing resistance control strategy of C.suppressalis.