厚皮甜瓜种质蔓枯病抗性评价与遗传多样性分析

Identification of gummy stem blight resistance and analysis of genetic diversity in muskmelon germplasms

采用苗期蔓枯病接种和单核苷酸多态性(SNP)基因型分析对120份厚皮甜瓜高代自交系进行抗性评价与遗传多样性研究。结果显示:光皮类型厚皮甜瓜中高抗、中抗种质资源占比达11.11%,其抗性水平结构优于网纹甜瓜和哈密瓜。统计分析结果显示,21个SNP位点的PIC值为0.050~0.478,其中12个中度多态SNP位点的PIC值平均为0.37,表明甜瓜种质的多态性中等。群体结构与聚类分析结果表明,124份甜瓜种质资源可分成5大类群,各类群具有一定的独立性,且类群间存在明显的差异,遗传多样性丰富。

The gummy stem blight(GSB)resistance evaluation and genetic diversity study of 120 high generation inbred lines of muskmelons were conducted via inoculation at seedling stage and single nucleotide polymorphism(SNP)genotype analysis.The results showed that,the proportion of germplasm resources of smoothbark muskmelons with high and medium resistance to GSB was up to 11.11%,and the structure of resistance level was better than that of netted melon and Hami melon.The statistical analysis results showed that,the polymorphism information content(PIC)value of 21 SNP loci ranged from 0.050 to 0.478,and the average PIC value of 12 moderate polymorphic SNP loci was 0.37,indicating that the polymorphism of the melon germplasms was moderate.Results of population structure and clustering analysis showed that,the 124 melon germplasms could be classified into five major groups,and each group showed certain independence.Furthermore,there were obvious differences between different groups and the genetic diversity was abundant.