扶正解毒化瘀方对MDRPA感染肺炎大鼠血清炎症细胞因子水平的影响

Intervention of Fuzheng Jiedu Huayu Formula(扶正解毒化瘀方)on Serum Immune Inflammatory Cytokines in MDRPA-infected Rats

目的:观察扶正解毒化瘀方对多重耐药铜绿假单胞菌(MDRPA)感染肺炎大鼠血清炎症细胞因子水平的影响,分析其治疗机制。方法:将150只SD大鼠随机分为5组,每组30只,其中模型组、中药组、哌拉西林/他唑巴坦(TZP)组、中西结合组采用经口气管插管法制备MDRPA急性肺炎模型,空白组则以等量生理盐水代替菌液。造模后1 d、3 d、5 d,TZP组各有10只大鼠分别给予TZP腹腔注射,日3次,中药组各有10只大鼠分别给予扶正解毒化瘀方颗粒剂溶液(7 g/kg)灌胃2次/日,中西结合组各有10只大鼠分别给予上述两种药物干预。药物干预完毕后,每组每个时间点6只大鼠取材,采用酶联免疫吸附(ELISA)法检测血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-6、IL-10、γ干扰素(IFN-γ)水平。结果:①造模后3 d,与模型组比较,中药组和中西结合组血清TNF-α水平均显著降低(P<0.01)。②造模后3 d,与模型组比较,中药组、TZP组和中西结合组血清IL-1β水平均降低(P<0.05或P<0.01);造模后5 d,与模型组比较,TZP组和中西结合组血清IL-1β水平均明显降低(P<0.05)。③各组不同时间点血清IL-6和IL-10水平差异均无统计学意义(P>0.05)。④造模后1 d,与模型组比较,中西结合组血清IFN-γ水平明显升高(P<0.05)。结论:扶正解毒化瘀方可下调MDRPA感染肺炎大鼠血清IL-1β、TNF-α表达,并上调IFN-γ表达,此可能为该方减轻炎症反应、促进机体免疫炎性损伤修复的机制之一。

Objective:To observe the effect of Fuzheng Jiedu Huayu Formula(扶正解毒化瘀方,FZJDHYF)on serum inflammatory cytokines of rats infected with pneumonia by multidrug resistant pseudomonas aeruginosa(MDRPA),and analyze the therapeutic mechanism of FZJDHYF.Methods:Total of 150 rats were randomly divided into 5 groups with 30 rats in each group.Model group,traditional Chinese medicine(TCM)group,Piperacillin/Tazobactam(TZP)group and TCM+TZP group(Combined group),were used to prepare MDRPA acute pneumonia model by otracheal intubation method,while blank group was replaced with the same amount of normal saline.At 1 d,3 d and 5 d after modeling,10 rats in the TZP group were given intraperitoneal injection of TZP 3 times/day,10 rats in the TCM group were given granular solution of FZJDHYF(7 g/kg)2 times/day,and 10 rats in the integrated Chinese and Western medicine group were given the above two medicines interventions.After drug intervention,six rats in each group were sampled at each time point were detected by ELISA,include TNF-α,IL-1β,IL-6,IL-10 and IFN-γ.Results:①Three days after modeling,compared with model group,the serum TNF-αlevel in TCM group and combined group was significantly decreased(P<0.01).②At 3 days after modeling,compared with model group,serum IL-1βlevel in TCM group,TZP group and combined group decreased(P<0.05 or P<0.01);On day 5 after modeling,compared with model group,serum IL-1βlevel in TZP group and was significantly decreased(P<0.05).③The difference in serum IL-6 and IL-10 levels among all groups at different time points was not statistically significant(P>0.05).④On day 1 after modeling,compared with model group,serum IFN-γlevel in combined group was significantly increased(P<0.05).Conclusions:FZJDHYF down-regulates the expressions of serum IL-1βand TNF-αin rats with MDRPA-infected pneumonia,and up-regulates the expression of IFN-γ,which may be one of the mechanisms of this formula to reduce inflammatory response and promote the repair of immune inflammatory injury.