DEAD盒多肽43小干扰RNA对司美替尼抑制人肺腺癌A549细胞增殖和诱导其凋亡作用的影响

Effect of DEAD box polypeptide 43 small interfering RNA on inhibition of human lung adenocarcinoma A549 cell proliferation and induction of cell apoptosis by simetinib

目的探讨DEAD盒多肽43(DDX43)小干扰RNA(siRNA)对司美替尼抑制人肺腺癌A549细胞增殖和诱导其凋亡作用的影响。方法培养A549细胞,并分为对照组、司美替尼组和司美替尼+DDX43 siRNA组,运用细胞免疫组化和原位杂交的方法分别检测各组A549细胞中DDX43蛋白和mRNA的表达情况,采用MTT法检测各组细胞的增殖情况,采用TUNEL法检测各组细胞的凋亡情况。结果对照组、司美替尼组、司美替尼+DDX43 siRNA组DDX43蛋白相对表达量分别为138.20±17.72、79.95±8.96、36.16±6.31,总体比较差异有统计学意义(F=66.72,P=0.02);表达水平依次降低,且两两比较差异均有统计学意义(P均<0.05)。对照组、司美替尼组、司美替尼+DDX43 siRNA组DDX43 mRNA相对表达量分别为266.20±15.07、164.95±8.96、71.16±6.31,总体比较差异有统计学意义(F=125.35,P=0.01);表达水平依次降低,且两两比较差异均有统计学意义(P均<0.05)。对照组、司美替尼组、司美替尼+DDX43 siRNA组A549细胞凋亡指数分别为(3.75±0.55)%、(11.72±1.06)%、(19.98±1.21)%,总体比较差异有统计学意义(F=12.31,P=0.04);凋亡指数依次升高,且两两比较差异均有统计学意义(P均<0.05)。对照组、司美替尼组、司美替尼+DDX43 siRNA组A549细胞生长抑制率分别为(2.11±0.13)%、(14.91±0.29)%、(27.14±0.58)%,总体比较差异有统计学意义(F=15.23,P=0.03);生长抑制率依次升高,且两两比较差异均有统计学意义(P均<0.05)。结论DDX43 siRNA联合司美替尼可抑制A549细胞增殖,促进其凋亡。

Objective To study the Effect of DEAD box polypeptide 43(DDX43)small interfering RNA(siRNA)on inhibition of human lung adenocarcinoma A549 cell proliferation and induction of cell apoptosis by simetinib.Methods A549 cells were cultured,and were divided into three groups:the control group,the selumetinib group,the selumetinib+DDX43 siRNA group.Immunochemical method and in situ hybridization were used to detect the DDX43 protein and mRNA,MTT method was used to detect the cell the proliferation,and TUNEL method was used to detect the cell apoptosis.Results The DDX43 protein relative expression levels were respectively 138.20±17.72,79.95±8.96,36.16±6.31 in the control group,the selumetinib group,the selumetinib+DDX43 siRNA group(F=66.72,P=0.02);the expression level decreased in turn(P<0.05).The DDX43 mRNA relative expression levels were respectively 266.20±15.07,164.95±8.96,71.16±6.31 in the control group,the selumetinib group,the selumetinib+DDX43 siRNA group(F=125.35,P=0.01);the expression level decreased in turn(P<0.05).The A549 cell growth inhibition rate were respectively(3.75±0.55)%,(11.72±1.06)%,(19.98±1.21)%in the control group,the selumetinib group,the selumetinib+DDX43 siRNA group(F=12.37,P=0.04);the growth inhibition rate increased in turn(P<0.05).The A549 cell growth inhibition rate were respectively(2.11±0.13)%,(14.91±0.29)%,(27.14±0.58)%in the control group,the selumetinib group,the selumetinib+DDX43 siRNA group(F=15.23,P=0.03);the growth inhibition rate increased in turn(P<0.05).Conclusion DDX43 siRNA combined with selumetinib can inhibit the proliferation of A549 cells,and promote the cell apoptosis.