取食不同寄主植物的灰飞虱对药剂敏感性和解毒酶活性的影响

Effects of host plants on insecticide sensitivity and detoxification enzyme activities in Laodelphax striatellu s(Hemiptera:Delphacidae)

为了探索取食不同寄主植物的灰飞虱Laodelphax striatellus对药剂敏感性和体内解毒酶活性的影响,本研究在室内采用稻苗浸渍法分别测定了4种杀虫剂对取食水稻、小麦和稗草后的灰飞虱3龄若虫的毒力,同时比较了灰飞虱体内酯酶(ESTs)、谷胱甘肽-S-转移酶(GSTs)和多功能氧化酶(MFO)的活性。结果表明,用5种寄主植物连续饲养灰飞虱3代后,灰飞虱对三氟苯嘧啶的敏感性无明显变化,对吡虫啉、噻虫嗪和溴氰虫酰胺的敏感性存在一定差异但不显著。取食不同寄主植物的灰飞虱体内3种解毒酶活性存在明显差异,取食稗草的灰飞虱体内ESTs和MFO活性最高,取食水稻紫香糯2315的灰飞虱体内GSTs活性最高。研究结果表明取食不同寄主植物可影响灰飞虱对杀虫剂的敏感性和体内解毒酶的活性。

In order to evaluate the effects of host plants on insecticide sensitivities and detoxification enzyme activities in the small brown planthopper(SBPH),Laodelphax striatellus,the toxicity of four insecticides to the 3 rd instar nymphs of SBPH fed on rice,wheat,and barnyard grass was determined by rice seedlings dipping method in the laboratory,respectively.The activities of esterase(ESTs),glutathione-S-transferase(GSTs)and mixed-function oxidase(MFO)of SBPH were also measured.The results showed that after reared for three generations on different host plants,there were no significant differences on the sensitivity of SBPH to triflumezopyrim,and the sensitivity to imidacloprid,thiamethoxam and cyantraniliprole were different but with no significance.The activities of the three detoxification enzymes showed differences after fed on the five host plants.The activities of ESTs and MFO in SBPH fed on barnyard grass were the highest and the activities of GSTs in SBPH fed on rice Zixiangnuo 2315 were the highest.These results indicated that SBPH reared on different host plants could induce different insecticide sensitivity and the activities of detoxifying enzymes.