四种鳃金龟对不同植物挥发物的触角电位反应

Electrophysiological responses of four scarab beetles to the volatiles produced by different plants

华北大黑鳃金龟Holotrichia oblita Falderman、暗黑鳃金龟Holotrichia parallela Motschulsky、小黄鳃金龟Metabolus flavescens Brenske和福婆鳃金龟Brahmina faldermanni Kraatz有不同的寄主范围。本研究采用溶剂提取法收集寄主植物玉兰Magnolia denudata Desr.、柿树Diospyros kaki L.f.、金银木Lonicera maackii(Rupr.)Maxim、榆树Ulmus pumila L.、芙蓉葵Hibiscus moscheutos L.、丁香Syzygiumr aromaticum(L.)Merrill&Perry和非寄主植物银杏Ginkgo biloba L.等7种植物叶片粗提物,分别测定4种金龟甲雄、雌虫对这些植物粗提物的触角电位反应。结果表明,4种金龟对共同寄主植物金银木和榆树粗提物均有较强的EAG反应;华北大黑鳃金龟雄虫对寄主植物粗提物的EAG反应均弱于雌虫;小黄鳃金龟雌虫对芙蓉葵粗提物的EAG反应强于雄虫;而另外两种金龟雄、雌虫对同种植物粗提物的EAG反应无显著差异。上述结果说明金龟甲的EAG反应大小与田间发生情况一致。

Four scarab beetles,Holotrichia oblita Falderman,Holotrichia parallela Motschulsky,Metabolus flavescens Brenske and Brahmina faldermanni Kraatz,have different plant host ranges.This study aims to test electrophysiological responses of male and female beetles to solvent extracts of leaves from host plants,Magnolia denudata Desr.,Diospyros kaki L.f.,Lonicera maackii(Rupr.)Maxim,Ulmus pumila L.,Hibiscus moscheutos L.,Syzygiumr aromaticum(L.)Merrill&Perry,and non-host plant Ginkgo biloba L.The results showed that four scarab beetles had strong EAG responses to host plant extracts of L.maackii and U.pumila.The EAG response of male H.oblita to plant extract was weaker than that of female beetles.The EAG response of female M.flavescens to H.moscheutos was stronger than that of male beetles.There was no significant sexual difference in the EAG response of the other two beetles to the same plant extracts.These results suggest that the strength in EAG responses of different scarab beetles is consistent with their occurrence on different host plants.