HPLC测定大风艾中5种二氢黄酮类成分的含量

Simultaneous Determination of Five Flavanones in Blumea Balsamifera by HPLC

目的测定大风艾药材中3,3’,5,7-四羟基-4’-甲氧基二氢黄酮、3’,5,5’,7-四羟基二氢黄酮、3,3’,4’,5-四羟基-7-甲氧基二氢黄酮、3,3’,5-三羟基-4’,7-二甲氧基二氢黄酮、艾纳香素5种二氢黄酮类成分的含量。方法采用高效液相色谱法测定,色谱柱为AKZONOBEL Kromasil 100-5C_(18)(4.6 mm×250 mm,5μm);流动相为乙腈(A)-0.2%冰醋酸(B),梯度洗脱(0~10 min,20%A;10~15 min,20%→25%A;15~25 min,25%A;25~50 min,25%→65%A);流速:1.0 mL·min^(-1);检测波长:289 nm;柱温:35℃;进样量:10μL。结果 5种二氢黄酮类成分的线性关系良好,加样回收率为98.73%~101.84%。结论该方法简便、准确,可用作大风艾药材质量控制的方法。

OBJECTIVE To determine content of 5 flavanones of 3,3’,5,7-tetrahydroxy-4’-methoxyflavanone,3’,5,5’,7-tetrahydroxy-flavanone,3,3’,4’,5-tetrahydroxy-7-methoxyflavanone,3,3’,5-trihydroxy-4’,7-dimethoxyflavanone,5-blumeatin in Blumea balsamifera.METHODS The chromatographic separation was achieved on AKZONOBEL Kromasil 100-5C18(4.6 mm×250 mm,5μm)column with the mobile phase consisting of acetonitrile(A)-0.2%HAc(B)by gradient elution program(0−10 min,20%A;10−15 min,20%→25%A;15−25 min,25%A;25−50 min,25%→65%A)at the flow rate of 1.0 mL·min^(−1),detection wavelength was set at 289 nm,and the column temperature was 35℃,and the injection volume was 10μL.RESULTS Linearity relationships of 5 flavanones were good,and the average recoveries were from 98.73%to 101.84%.CONCLUSION The method is simple,accurate and repeatable and it is suitable for quality control of Blumea balsamifera.