乙型肝炎病毒核酸检测在抗病毒治疗中的应用价值

Application value of hepatitis B virus nucleic acid testing in antiviral therapy

乙型肝炎(乙肝)病毒(HBV)感染是导致乙肝肝硬化和原发性肝细胞癌(HCC)发生的主要因素之一。目前临床上使用抗病毒药物抑制HBV的复制,但抗病毒药物耐药性的发生很难被及时发现,因此HBV核酸检测(NAT)作为预测HBV耐药性发生的主要手段,在慢性乙肝患者诊断及抗病毒治疗的应用中不可或缺。本文从乙肝DNA定量检测、HBV基因P区检测和基因分型、微小RNA(miRNA)检测等方面进行综述,分析NAT在HBV耐药性诊断以及HCC癌变检测中的应用,讨论NAT对减少乙肝抗病毒药物耐药性和降低HCC在慢性乙肝患者中的发病率的价值。

Hepatitis B virus(HBV)infection was one of the main factors leading to hepatitis B cirrhosis and hepatocellular carcinoma(HCC).At present,antiviral drugs were used to inhibit the replication of HBV in clinic.However,it was difficult to detect the occurrence of antiviral drug resistance.Therefore,HBV nucleic acid testing(NAT),as the main means for the prediction of HBV resistance,was indispensable method in the diagnosis and application of antiviral therapy in patients with chronic hepatitis B.This article reviewed the quantitative detection of hepatitis B DNA,detection of HBV gene P region and genotyping,and microRNA(miRNA)detection.It also analyzed the application of NAT technology in the diagnosis of HBV resistance and detection of HCC carcinogenesis,and discusses the value of this technology in decreasing the resistance of HBV and reducing the incidence of HCC in chronic hepatitis B patients.