浓缩生长因子纤维蛋白膜的制备及降解性能

Preparation and degradation properties of concentrated growth factor fibrin membrane

背景:在引导骨再生手术临床应用中生物材料的降解时间至关重要。目的:系统比较富血小板纤维蛋白膜与浓缩生长因子纤维蛋白膜体外降解性能的差异,并分析同种型号不同机器与不同采血管对浓缩生长因子纤维蛋白膜降解性能的影响。方法:利用Multi-CGF-3离心机分别制备富血小板纤维蛋白膜与浓缩生长因子纤维蛋白膜,然后将两种纤维蛋白膜分别浸泡于唾液中,观察膜质量与面积的变化。采用同种型号的两台离心机分别制备浓缩生长因子纤维蛋白膜,浸泡于唾液中观察膜质量与面积的变化。采用两种真空采血管(BD管、康捷管)分别制备浓缩生长因子纤维蛋白膜,浸泡于唾液中观察膜质量与面积的变化。结果与结论:①两种纤维蛋白膜的体外降解性能存在差异,浓缩生长因子纤维蛋白膜初始质量更大且体外降解时间更长;②同种型号不同机器制备的浓缩生长因子纤维蛋白膜初始质量无明显差异,初始面积质量比越小的浓缩生长因子纤维蛋白膜降解速度越慢;③与康捷管相比,BD管离心后的血液样本分层效果较好,但两种试管制备的浓缩生长因子纤维蛋白膜的降解时间相近,总体降解性能相似;④结果表明,作为最新一代的自体血小板浓缩制品,浓缩生长因子纤维蛋白膜展现了较富血小板纤维蛋白膜更优异的降解性能。

BACKGROUND:The degradation time of biomaterials is crucial in the clinical application of guided bone regeneration surgery.OBJECTIVE:To systematically compare the difference in degradation performance of platelet-rich fibrin membrane and concentrated growth factor membrane in vitro,and analyze the effects of different machines of the same type and different blood collection tubes on the degradation performance of concentrated growth factor membrane.METHODS:Platelet-rich fibrin membranes and concentrated growth factor membranes were prepared with a Multi-CGF-3 centrifuge,and soaked in saliva to observe the changes in membrane quality and area.Two centrifuges of the same type were used to prepare concentrated growth factor membranes,which were then soaked in saliva to observe changes in membrane quality and area.Two types of vacuum blood collection tubes(BD tube and Kangjie tube)were used to prepare concentrated growth factor membranes,and the membranes were soaked in saliva to observe changes in membrane quality and area.RESULTS AND CONCLUSION:(1)The in vitro degradation performance of the platelet-rich fibrin membrane and concentrated growth factor membrane was different.The initial mass of the concentrated growth factor membrane was larger and its degradation time in vitro was longer.(2)The initial mass of the concentrated growth factor membranes prepared by different machines of the same type had no significant difference.The smaller the initial area-to-mass ratio,the slower the degradation rate of the concentrated growth factor membrane.(3)Compared with the Kangjie tube,the BD tube had a better layering effect on blood samples after centrifugation;however,the degradation time and overall degradation performance of concentrated growth factor membranes prepared by the two test tubes were similar.(4)The results show that,as the latest generation of autologous platelet-concentrated products,the concentrated growth factor membrane exhibits better degradation performance than the platelet-rich fibrin membrane.