基于多元统计分析的车前子药材质量等级研究

Study on Quality Grade of Plantaginis Semen Based on Multivariate Statistical Analysis

目的以市场收集的48批车前子药材为研究对象,构建外观性状与内在质量相关联的车前子的质量等级评价手段。方法建立了车前子中京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷的含量测定方法并完成48批次的测定,同时对48批车前子药材进行了千粒重、长度、宽度、膨胀度、水分、总灰分、酸不溶性灰分的检测;运用主成分分析、偏最小二乘法分析、相关性分析、方差分析等多种数理统计手段,筛选分级指标进行车前子等级划分研究。结果经筛选认为千粒重、膨胀度、京尼平苷酸含量、毛蕊花糖苷与异毛蕊花糖苷总含量为车前子品质评价的关键指标,且外在指标(千粒重、膨胀度)与内在质量(京尼平苷酸含量、毛蕊花糖苷与异毛蕊花糖苷总含量)在0.01水平上呈显著性正相关,结合市场实际情况,制定了车前子一等、二等、统货的质量等级标准。结论该质量等级不仅从外观特性上对车前子进行了等级评价,同时也在其内在质量差异上进行了表征,满足了历代对于车前子“饱满者为佳”的判断规律,也为行业标准或国家标准的建立提供依据。

OBJECTIVE To construct a quality grade evaluation method of plantain seed based on 48 batches of Plantaginis Semen collected from the market.METHODS Determination method of the contents of geniposidic acid,acteoside and isoacteoside in Plantaginis Semen was established and 48 batches herbs were determined.At the same time,1000-grain weight,length,width,swelling degree,water content,total ash content and acid insoluble ash content of 48 batches of Plantaginis Semen herbs were tested.The principal component analysis,partial least square analysis,correlation analysis,variance analysis and other mathematical statistical methods were used to select the classification index for the study of Plantaginis Semen classification.RESULTS The 1000-grain weight,swelling degree,geniposidic acid content,total contents of acteoside and isoacteoside were the key indicators of the quality evaluation of Plantaginis Semen,moreover,external index(1000-grain weight,swelling degree)and inner quality(geniposidic acid content,total contents of acteoside and isoacteoside)at the 0.01 level showed significant positive correlation,in combination with the practical situation of the market,the Plantaginis Semen quality grade standard of first class,second class and uniform goods were developed.CONCLUSION This quality grade not only evaluates Plantaginis Semen in terms of appearance characteristics,but also characterizes its internal quality difference,which satisfies the judgment rule of“plump seed is the best”and provides basis for the establishment of industry standard or national standard.