实时荧光PCR检测腹泻患者肠致泻性大肠杆菌的分布情况研究

Study on the distribution of enterovirulent E.coli detected by real-time fluorescent PCR in patients with diarrhea

目的研究实时荧光PCR检测腹泻患者肠致泻性大肠杆菌的分布情况。方法150例腹泻患者粪便标本,应用实时荧光PCR技术对其病原菌进行检测,并分析不同类型病原菌在腹泻患者中的分布情况。结果150例腹泻粪便样本中共检出致泻性大肠杆菌36例,总检出率为24.00%,其中检出肠致病性大肠杆菌占比50.00%,肠黏附性大肠杆菌占比33.33%;肠出血性大肠杆菌占比8.33%;肠产毒性大肠杆菌占比5.56%,肠侵袭性大肠杆菌占比2.78%。10月致泻性大肠杆菌的检出率为25.93%,11月和12月致泻性大肠杆菌检出率分别为26.00%和19.57%,以11月检出率较高。从年龄分布情况来看,<5岁患者的致泻性大肠杆菌、肠致病性大肠杆菌检出率相对较高。结论腹泻患者的肠致泻性大肠杆菌以肠致病性大肠杆菌、肠黏附性大肠杆菌为主。

Objective To study the distribution of enterovirulent E.coli detected by real-time fluorescent PCR in patients with diarrhea.Methods Stool specimens of 150 patients with diarrhea were detected by realtime fluorescent PCR technology,and the distribution of different types of pathogens in patients with diarrhea was analyzed.Results A total of 36 cases of diarrheal E.coli were detected in 150 stool samples of diarrhea,with a total detection rate of 24.00%,of which enteropathogenic E.coli accounted for 50.00%,and entero-adhesive E.coli accounted for 33.33%;intestinal bleeding E.coli accounted for 8.33%;enterotoxigenic,E.coli accounted for 5.56%,and enteroinvasive E.coli accounted for 2.78%.The detection rate of enterovirulent E.coli in October was 25.93%,which were 26.00%and 19.57%,respectively in November and December.The detection rate was higher in November.According to the age distribution,the detection rate of enterovirulent E.coli and enteropathogenic E.coli in patients<5 years old is relatively high.Conclusion Enterovirulent E.coli in patients with diarrhea is mainly enteropathogenic E.coli and entero-adhesive E.coli.