唾液菌群微生物与宿主血脂水平的关联分析

Correlation analysis of salivary microbiome and host blood lipid levels

目的·分析唾液菌群微生物与宿主血脂水平的相关性。方法·采集114名45~60岁志愿者的唾液菌群样本,提取总DNA,随后应用多聚酶链式反应进行16S rDNA V3-V4区扩增。扩增所得产物经Illumina MiSeq PE300平台测序后,将所得序列进行操作分类单元(operational taxonomic units,OTUs)聚类、物种注释并将唾液菌群中丰度前50的属与宿主血脂水平作Spearman相关分析,P<0.05记为差异有统计学意义。结果·114名志愿者的唾液菌群样本测序所得平均序列数为(41084±4740)条,聚类所得OTUs数为1153,共注释到23个门、43个纲、89个目、147个科和317个属。门水平和属水平的物种聚类热图示114个唾液菌群样本具有相似的物种组成丰度模式。Spearman相关分析结果显示,在唾液菌群微生物丰度前50的属中,Neisseria spp.的丰度与宿主血清总胆固醇(total cholesterol,TCH)、三酰甘油(triacylglycerol,TAG)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-Ch)的水平成负相关;Gemella spp.的丰度与宿主血清TCH、LDL-Ch水平呈正相关;与宿主血清高密度脂蛋白胆固醇水平成负相关的属有Comamonas spp.、Filifactor spp.和Parvimonas spp.。结论·人群中唾液菌群微生物的组成结构具有相对稳定性且与宿主血脂水平相关,可进一步挖掘唾液菌群微生物作为个体脂代谢异常的预警指标。

Objective·To analyze the correlation between salivary microbiome and host blood lipid levels.Methods·Samples of saliva from 114 volunteers aged 45-60 years were collected to extract total DNA,and then 16 S rDNA V3-V4 region was amplified with polymerase chain reaction.After the amplified products were sequenced via Illumina MiSeq PE300 platform,the obtained sequences were subjected to operational taxonomic units(OTUs)clustering and species annotation.The Spearman correlation analysis was also carried out to find the correlations between the top 50 abundant genera in the salivary microbiome and host blood lipid levels,P<0.05 was considered as statistically significant difference.Results·The saliva samples of 114 volunteers were sequenced with an average sequence number of 41084±4740,and the number of OTUs obtained by clustering was 1153,annotated to23 phyla,43 classes,89 orders,147 families and 317 genera.Species clustering heat map of 114 samples suggested that salivary microbiome has similar abundance patterns of species composition in the population.Spearman correlation analysis showed that among triacylglycerol(TAG)the top 50 abundant genera in the salivary microbiome,Neisseria spp.were negatively correlated with the host serum total cholesterol(TCH),and low density lipoprotein cholesterol level(LDL-Ch);Gemella spp.are positively correlated with Host serum TCH and LDL-Ch;high-density lipoprotein cholesterol presents negative correlation with Comamonas spp.,Filifactor spp.and Parvimonas spp.Conclusions·The composition structure of salivary microbiota in the population is quite stable and is related to the levels of host blood lipids.It is promising to further explore salivary microbiota as early warning indicators of individual lipid metabolism abnormalities.