结肠癌中长链非编码RNA LUCAT1水平与患者预后的关系及对结肠癌细胞迁移、侵袭的影响

Relationship between lncRNA LUCAT1 level in colon cancer and prognosis of patients and its effect on colon cancer cell migration and invasion

目的:分析结肠癌中长链非编码RNA(lncRNA)肺癌相关转录本1(LUCAT1)水平与患者预后的关系及对结肠癌细胞迁移、侵袭的影响。方法:选择2016年1月—2017年12月收治的行手术治疗的53例结肠癌患者作为研究对象,检测受试者肿瘤病灶组织和癌旁组织中lncRNA LUCAT1表达水平。取LoVo细胞检测分为空白组、阴性对照组、干预组,检测细胞增殖、迁移、侵袭活性。结果:肿瘤病灶组织中lncRNA LUCAT1水平明显高于癌旁组织,差异有统计学意义(P<0.05);肿瘤病灶组织中lncRNA LUCAT1水平是影响患者总生存期和无进展生存期的独立性威胁因素(P<0.05);低lncRNA LUCAT1表达患者总生存期和无进展生存期明显低于高lncRNA LUCAT1表达患者,差异有统计学意义(P<0.05);24 h和48 h时空白组、阴性对照组和干预组LoVo细胞增殖活性存在明显差异,其中空白组最高,干预组最低,差异有统计学意义(P<0.05);3组LoVo细胞迁移及侵袭活性存在明显差异,其中空白组迁移及侵袭活性最高,干预组最低,差异有统计学意义(P<0.05)。结论:结肠癌肿瘤病灶组织中高lncRNA LUCAT1是影响患者预后质量的独立性威胁因素,且可促进结肠癌细胞迁移、侵袭。

Objective: To analyze the relationship between the level of lncRNA LUCAT1 in colon cancer and the prognosis of patients and its impact on colon cancer cell migration and invasion. Methods: A total of 53 patients with colon cancer who underwent surgical treatment between January 2016 and December 2017 in our hospital were selected as the research object. The expression level of lncRNA LUCAT1 in the lesion tissue and adjacent tissue of the subject was detected. LoVo cell detection was divided into blank group, negative control group and intervention group, which were tested for cell proliferation, migration and invasion activity. Results: The level of lncRNA LUCAT1 in tumor tissue was significantly higher than that in adjacent tissues, and the difference was statistically significant(P<0.05). The level of lncRNA LUCAT1 in tumor tissue was an independent threat factor that affected the overall survival and progression-free survival of patients(P<0.05). The overall survival and progression-free survival of the patients with low lncRNA LUCAT1 expression were significantly lower than those with high lncRNA LUCAT1 expression, and the difference was statistically significant(P<0.05). There were significant differences in the proliferation activity of LoVo cells among the blank group, negative control group and intervention group at 24 h and 48 h, among which the blank group was the highest and the intervention group was the lowest, and the difference was statistically significant(P<0.05). There were obvious differences in LoVo cell migration and invasion activity among the blank group, negative control group and intervention group, among which the blank group had the highest migration and invasion activity, and the intervention group had the lowest, and the difference was statistically significant(P<0.05). Conclusion: High lncRNA LUCAT1 in colon cancer lesion tissue might be an independent threat factor that could affect the prognostic quality of patients, and could promote colon cancer cell migration and invasion.