MED28在口腔鳞状细胞癌及癌旁组织中的表达及临床特征分析

Expression of MED28 in oral squamous cell carcinoma and para-carcinoma tissues and its correlation with clinical characteristics

目的探讨中介体复合物亚基28蛋白(mediator complex subunit 28 protein,MED28)于口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)、癌旁组织中表达及其与病理特征、预后的关系。方法选取2015年1月至2020年6月首次在我院口腔颌面外科住院手术的OSCC患者标本142例,其中91份OSCC标本包含癌旁正常组织。免疫组织化学法于OSCC及癌旁组织石蜡切片检测MED28的表达。分析OSCC组织与癌旁组织的MED28表达差异。计算MED28表达结果即切片最终评分的平均值,并以此为标准将OSCC患者分为MED28低表达组和MED28高表达组,用Logistic回归模型分析MED28表达与病理特征的关系,Kaplan-Meier法分析MED28的表达与预后的关系,并用COX回归模型分析预后的影响因素。结果与癌旁组织比较,MED28在OSCC组织中高表达(P<0.01)。Logistic回归模型分析显示MED28表达与吸烟、淋巴结转移相关(P<0.05),Kaplan-Meier生存分析显示MED28低表达组总体生存时间(overall survival,OS)显著高于高表达组(P<0.01)。吸烟和性别也是影响预后的相关因素(P<0.05)。结论MED28在OSCC患者癌组织中高表达,可能与OSCC的发生、发展相关,并影响预后,其表达与吸烟、淋巴结转移有关。

Objective To investigate the expression of mediator complex subunit 28 protein(MED28)in oral squamous cell carcinoma(OSCC)and para-carcinoma tissues,and its correlation with pathological features and prognosis.Methods A total of 142 OSCC specimens were collected from the inpatients undergoing surgical treatment in our department from January 2015 to June 2020.Among these specimens,91 had para-carcinoma tissues.Immunohistochemistry was used to detect the MED28 expression in OSCC and para-carcinoma paraffin sections.Chi-square test was used to analyze the difference of MED28 expression between OSCC and para-carcinoma tissues.The patients were then divided into MED28-high and MED28-low expression groups according to its median expression level.Logistic regression model was used to analyze the relationship between MED28 expression and pathological features,Kaplan-Meier survival analysis was selected to study the relationship between the expression and prognosis,and COX regression analysis was employed to analyze the prognostic factors.Results MED28 was significantly highly expressed in the OSCC tissues than para-carcinoma tissues(P<0.01).Logistic regression model showed that MED28 expression is related with smoking and lymph node metastasis(P<0.05).Kaplan-Meier survival analysis indicated that overall survival(OS)in the patients was significantly higher in the MED28-low expression group than the MED28-high expression group(P<0.01).Gender and smoking status were identified as related factors influencing prognosis(P<0.05).Conclusion MED28 is highly expressed in the OSCC tissues,which might be related to the occurrence and development of OSCC.It also affects the prognosis,and is related to smoking status and lymph node metastasis.