基于黄酮组分探讨黄芪葛根配伍对糖尿病大鼠脂肪组织细胞因子的调控作用

Based on Flavonoids Components to Study on Regulating Effects of Astragali-Pueraria lobata Compatibility on Cytokines in Diabetic Rats Adipose Tissue

目的探讨黄芪黄酮、葛根黄酮配伍对糖尿病大鼠脂肪组织细胞因子的影响,明确两者配伍对血糖血脂的调控机制。方法将66只SPF级雄性SD大鼠随机分为6组,分别为正常组、模型组、对照组、黄芪黄酮组、葛根黄酮组、黄酮配伍组,每组11只大鼠。除正常组外,其余各组一次性腹腔注射2%STZ(46mg·kg^(-1)),造模当日灌胃给药,对照组、黄芪黄酮组、葛根黄酮组、黄酮配伍组分别予金芪降糖片混悬液1.47g·kg^(-1)、黄芪黄酮0.039g·kg^(-1)、葛根黄酮0.036g·kg^(-1)、黄芪黄酮+葛根黄酮0.075g·kg^(-1) 30天。酶联免疫吸附测定(ELISA)检测脂联素(APN)、脂联素受体(AdipoRs)、瘦素(LEP)、瘦素受体(LepR)、肿瘤坏死因子α(TNF-α)、白介素-12(IL-12)、白介素-15(IL-15),实时荧光定量聚合酶链式反应法(Real-time PCR)检测Chemerin、ChemR23 mRNA。结果与正常组比较,模型组APN、AdipoRs、LEP、LepR降低,TNF-α、IL-12、IL-15、Chemerin、ChemR23 mRNA升高(P<0.05)。与模型组比较,黄芪黄酮、葛根黄酮、黄芪黄酮与葛根黄酮配伍能够升高糖尿病大鼠APN(黄芪黄酮、葛根黄酮除外)、AdipoRs、LepR含量,降低TNF-α、IL-12、IL-15、Chemerin(葛根黄酮、黄酮配伍除外)、ChemR23 mRNA表达(P<0.05)。结论黄芪黄酮与葛根黄酮配伍通过促进脂肪组织APN、AdipoRs表达,降低TNF-α、IL-12、IL-15含量,调控糖尿病大鼠血糖血脂。

Objective To explore the regulatory effects of Astragali flavonoids and Pueraria lobate flavonoids compatibility on cytokines in adipose tissue, the mechanism of reducing blood glucose and lipids was revealed. Methods 66 SPF male SD rats were randomly divided into 6 groups: normal group, model group, control group, Astragalus Flavonoids group, Pueraria lobate Flavonoids group and Flavonoids compatibility group. There were 11 rats in each group. Except for the normal group, 2%STZ(46 mg·kg-1) was given to rats of the other groups by intraperitoneal injection. On the same time, 1.47·kg-1 Jinqi Jiangtang tablet suspension was given to rats in control group. 0.039 g·kg-1 Astragalus flavonoids was given to rats in Astragalus Flavonoids group. 0.036 g·kg-1Pueraria lobate flavonoids was given to Pueraria lobate Flavonoids group. 0.075 g·kg-1 Astragali flavonoid and Pueraria lobate flavonoid were given to Flavonoids compatibility group. All of drugs were given in 30 days continuously. The contents of adiponectin(APN), adiponectin receptors(AdipoRs), leptin(LEP), leptin receptors(LepR), tumor necrosis factor α(TNF-α), interleukin 12(IL-12) and interleukin 15(IL-15) were detected by enzyme-linked immunosorbent assay(ELISA). The relative expression of Chemerin and ChemR23 mRNA was tested by Real-time PCR. Results Compared with the normal group, the expression of APN, AdipoRs, LEP and LepR were lowered, while the expression of TNF-α, IL-12, IL-15, chemerin and chemR23 mRNA were increased in model rats(P<0.05). Astragalus flavonoids, Pueraria lobate flavonoids, and their compatibility could increase APN(except Astragalus flavonoids and Pueraria lobate flavonoids), AdipoRs, LEP and LepR of model rats(P<0.05). Astragalus flavonoids, Pueraria lobate flavonoids, and their compatibility could reduce TNF-α, IL-12, IL-15, Chemerin(except Pueraria lobate flavonoids, flavonoids compatibility) and ChemR23 mRNA(P<0.05). Conclusion The compatibility of Astragalus flavonoids and Pueraria lobate flavonoids could promote the expression of APN and AdipoRs, reduce the contents of TNF-α, IL-12 and IL-15 in adipose tissue. Which could regulate blood glucose and blood lipid in diabetic rats.