血清肝再生磷酸酶-3鉴别诊断高、低级别浆液性卵巢癌价值

Value of serum phosphatase of regenerating liver-3 for differential diagnosing high-grade and low-grade serous ovarian cancer

目的:分析血清肝再生磷酸酶-3(PRL-3)在高、低级别浆液性卵巢癌患者表达及其鉴别诊断价值。方法:选取2015年3月—2019年1月在本院手术治疗的浆液性卵巢癌106例为观察组,按美国M.D.Anderson肿瘤中心提出的分级系统,分为低级别浆液性卵巢癌45例(低级别组)与高级别浆液性卵巢癌61例(高级别组),另选同期本院体检健康女性98例为对照组。实时荧光定量PCR(qRT-PCR)检测所有研究对象血清PRL-3表达并比较;采用受试者工作特征曲线(ROC)预测PRL-3水平对高、低级别卵巢癌的诊断价值。结果:3组年龄、体质指数、糖尿病及高血压比例无差异(P>0.05),高级别组FIGO分期Ⅲ-Ⅳ期患者比例高于低级别组(P<0.05)。血清中PRL-3表达水平高级别组最高(2.61±0.72),低级别组次之(1.39±0.69),对照组最低(1.16±0.43)(P<0.05),但不同分期患者表达水平未见差异。血清中PRL-3水平诊断高、低级别浆液性卵巢癌的ROC曲线下面积为0.887(95%CI:0.811~0.940),截断值为1.95,灵敏度为90.2%,特异性为77.8%。结论:浆液性卵巢癌患者血清PRL-3表达上调,且高级别患者高于低级别患者,PRL-3对鉴别诊断高、低级别浆液性卵巢癌有一定价值。

Objective:To analyze the expression of phosphatase of regeneration of liver-3(PRL-3)in serum of women with high and low grade serous ovarian cancer,and to analyze its differential diagnostic value of high and low grade serous ovarian cancer.Methods:106 women with serous ovarian cancer were selected in group A from March 2015 to January 2019,and according to the grading system proposed by the American M.D.Anderson Cancer Center,these women were divided into group A1(45 cases with low-grade serous ovarian cancer)and group A2(61 cases with high-grade serous ovarian cancer).In addition,98 healthy women were selected in group B during the same period.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the expression of serum PRL-3 of the women in the three groups,and the level of serum PRL-3 of the women was compared among the three groups.The receiver operating characteristic curve(ROC)was used to predict the value of PRL-3 level for diagnosing high or low serous ovarian cancer.Results:There were no significant differences in age,BMI,and the ratios of diabetes and high blood pressure of the women among the three groups(P>0.05).The ratio of the women with FIGO stagingⅢandⅣin group A2 was significant higher than that of the women in group A1(P<0.05).The expression level of PRL-3 in serum(2.61±0.72)of the women in group A2 was the highest,followed by that(1.39±0.69)of the women in group A1,and that(1.16±0.43)of the women in group B was the lowest(P<0.05),but there was no significant differences in the expression level of PRL-3 among the women with different FIGO staging.The area under the ROC curve,the cut-off value,the sensitivity,and the specificity of PRL-3 level for diagnosing high and low grade serous ovarian cancer were 0.887(95%CI:0.811-0.940),1.95,90.2%,and 77.8%,respectively.Conclusion:The expression of serum PRL-3 of women with serous ovarian cancer has up-regulated,and the expression of PRL-3 of women with high-grade serous ovarian cancer patients is significant higher than that of women with low-grade serous ovarian cancer.So the level of PRL-3 has certain differential diagnostic value for high-grade and low-grade serous ovarian cancer.