抗菌肽Chionodracine对痤疮丙酸杆菌抑菌及抗炎作用的研究

Inhibitory Effect of the Antimicrobial Peptide Chionodracine Against Propionibacterium Acnes and Its Anti-inflammatory Effect on Acne Vulgaris

痤疮丙酸杆菌了参与寻常痤疮的发病和炎症过程。本文以冰鱼抗菌肽Chionodracine为研究对象,在体外和体内对其抗痤疮丙酸杆菌和抗炎作用进行了研究。Chionodracine对痤疮丙酸杆菌的最小抑菌浓度为12.5μM;对热和pH有一定的抗性;对正常人表皮角质细胞无明显的毒性;对痤疮丙酸杆菌造成的大鼠耳廓肿胀具有较好的抑制作用。

Propionibacterium acnes is involved in the pathogenesis and inflammation of acne vulgaris.An antimicrobial peptide named Chionodracine,which was determined from icefish species Chionodracohamatus,has been shown to possess certain antibacterial activity.This study was carried out with synthesized Chionodracine on the basis of the antimicrobial and anti-inflammatory activities against P.acnes in vitro and in vivo.The minimal inhibitory concentration(MIC)of Chionodracine against P.acnes is 12.5μM.And Chionodracine exhibits certain resistance to heat and pH,but no significant cytotoxicity to normal human epidermal keratinocytes(NHEKs).Using the rat ear edema model,Chionodracine significantly suppressed P.acnes-induced skin inflammation.