灵芝多糖提取及纯化工艺研究

Study on Extraction and Purification Technology of Ganoderma Lucidum Polysaccharide

为了优化制备高纯度灵芝多糖的提取及纯化工艺,获得高纯度的灵芝多糖药学研究用样品。以灵芝子实体为原料,药用纯化水为溶剂,以固形物含量和多糖含量为考察指标,通过对比研究确定灵芝水提物生产的最优工艺条件。灵芝水提物制备的最佳工艺条件为:料液比1︰15、提取两次、第一次提取时间为4 h、第二次提取时间为3 h,得到水提物收率6.54%,多糖含量9.1%。灵芝水提物通过分级醇沉方法纯化分离灵芝多糖,以多糖含量为考察指标,对比两组不同梯度的体积分数醇沉工艺,确定灵芝多糖分级醇沉的的最佳工艺条件。灵芝水提物分级醇沉的最优工艺条件为30%、60%和90%三个醇沉体积分数,通过分级醇沉可以将水提物中80%以上的灵芝多糖纯化分离出来,其中60%、90%体积分数醇沉得到的灵芝多糖2#、灵芝多糖3#的多糖含量达到25%以上。

In order to obtain samples for pharmaceutical research of Ganoderma polysaccharides, Ganoderma fruiting bodies were used as material, purified water as solvent, solid content and polysaccharide content as evaluation indexes. Through the contrastive research, we determined the optimal technological conditions for the production of water extract of Ganoderma as follows: material-liquid ratio of 1︰15, extraction twice, the first extraction time of 4 hours, and the second time of 3 hours. Then the yield of water extract was 6.54 %, and the content of polysaccharide was 9.1 %. The water extract was purified and separated by grading ethanol precipitation to prepare Ganoderma polysaccharide extract. Taking the content of polysaccharide as the index, comparing two groups of different gradient volume fraction ethanol precipitation, the optimal process conditions of grading ethanol precipitation were determined as three volume fractions which were 30 %, 60 % and 90 %. According to the above method, more than 80 % of Ganoderma polysaccharide could be attained. The polysaccharide content of the Ganoderma Polysaccharide 2# and the Ganoderma Polysaccharide 3#, which were separated from the 60 % and 90 % volume frations, could reach more than 25 %.