摘要
以无水乙醇作为溶剂,在检测波长274 nm的条件下,建立了一种用紫外-可见分光光度计快速检测辅酶Q10含量的方法。以辅酶Q10标准品质量浓度(C)为横坐标,以不同浓度标准品的吸光度测量值(A)为纵坐标建立标准曲线,得线性回归方程为:y=0.0251x-0.0053,R2=0.9996,在1.02~25.50μg/mL范围内呈现良好的线性关系。且该方法具有精密度高、稳定性和重现性好等优点。
Anhydrous ethanol as solvent, detection wavelength at 274 nm, a method for measuring the content of coenzyme Q10was established by UV-vis spectrophotometry. Using the standard quality concentration of Coenzyme Q10(C) as the abscissa, and the absorbance measurement value(A) of the standards of different concentrations as the ordinate to establish a standard curve, the linear regression equation was y=0.0251 x-0.0053, R2=0.9996. It shows a good linear relationship in the range of 1.02~25.50 μg/mL. This method has the advantages of high precision, good stability and reproducibility.
作者
邓乾华
杨善彬
李想
冯婷婷
张雪华
夏艳
陈荣彬
廖友林
陶桂英
王晓楠
苟文琼
唐佳丽
李倩蘭
李霄
蒲道俊
Deng Qianhua;Yang Shanbin;Li Xiang;Feng Tingting;Zhang Xuehua;Xia Yan;Chen Rongbin;Liao Youlin;Tao Guiying;Wang Xiaonan;Gou Wenqiong;Tang Jiali;Li Qianlan;Li Xiao;Pu Daojun(College of Chemistry,Chongqing Normal University,Chongqing 401331;Engineering Research Center for Biotechnology of Active Substances,Ministry of Education,Chongqing Normal University,Chongqing 401331;Chongqing Enterprise Key Laboratory of Sustained and Controlled Release Preparations of Southwest Pharmaceutical Co.,Ltd.,Chongqing 400038,China)
出处
《广东化工》
CAS
2021年第5期173-174,184,共3页
Guangdong Chemical Industry
基金
重庆市高校创新团队项目(CXTDX201601018)
活性物质生物技术教育部工程研究中心开放基金项目(No.20150408,AS201903)
重庆师范大学成果转化项目基金(15XZH08)
重庆师范大学博士启动项目(12XLB006)
重庆市大学生创新创业训练计划项目(S201910637014,S202010637012)
重庆市社会事业与民生保障科技创新专项项目(cstc2015shmszx80060)。
