原花青素对乙二醇所致大鼠肾结石的影响

The Effect of Procyanidins on Kidney Stones Induced by Ethylene Glycol in Rats

目的:探究肾结石大鼠在原花青素的干预下,其相关血液指标、肾内结晶及肾小管损伤情况,为预防肾结石提供思路。方法:40只健康雄性SD大鼠随机分为空白组(A)、成石组(B)、低、高剂量原花青素干预组(C、D)各10只。除空白组外,均采用1.25%乙二醇合1%氯化铵灌胃法制作肾结石大鼠模型,C、D组分别用原花青素5 mg/kg、10 mg/kg灌胃。实验结束时检测大鼠血肌酐、尿素氮浓度及肾组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量并观察大鼠肾内结晶情况及肾小管氧化应激程度。结果:成石组与空白组比较,大鼠肌酐、尿素氮及MDA含量增加,SOD活性降低(P<0.01);低、高剂量原花青素干预组大鼠经不同浓度原花青素处理后与成石组比较,大鼠肌酐、尿素氮及MDA含量降低,SOD活性增加(P<0.05)。经原花青素干预后,肾小管内形成晶体数量减少,且以高剂量原花青素(10 mg/kg)灌胃后减少更明显,且肾小管炎性反应减轻更明显。结论:肾结石大鼠在原花青素的干预下,肾内氧化应激水平降低,肾功能转好,肾内结晶数量减少,证明原花青素对预防大鼠肾结石及保护肾功能有积极作用。

Objective: To explore the blood indexes, intra-renal crystallization and renal tubular damage in rats with kidney stones under the intervention of proanthocyanidin, and to provide ideas for the prevention of kidney stones. Methods: Forty healthy male SD rats were randomly divided into blank group(A),stone-forming group(B), low-dose and high-dose proanthocyanidin intervention groups(C, D) each with 10 rats. Except for the blank group, the rats model of renal calculus were made by intragastric administration of 1.25 % ethylene glycol and 1 % ammonium chloride. Groups C and D were intragastrically administered with proanthocyanidins 5 mg/kg and 10 mg/kg, respectively. At the end of the experiment, the blood creatinine, urea nitrogen concentration, superoxide dismutase(SOD)activity and malondialdehyde(MDA) content in the kidney tissue were detected, and the crystallization in the rat kidney and the degree of renal tubular oxidative stress were observed. Results: Compared with the blank group, the contents of creatinine, urea nitrogen and MDA of rats increased, and SOD activity decreased(P<0.01);the rats in the low and high dose procyanidin intervention group were treated with different concentrations of proanthocyanidin compared with the stone group,Rat creatinine, urea nitrogen and MDA content decreased, SOD activity increased(P<0.05). After the intervention of proanthocyanidin, the number of crystals formed in the renal tubules decreased, and the decrease was more obvious after high-dose proanthocyanidin(10 mg/kg) gavage, and the renal tubular inflammatory reaction was alleviated more significantly. Conclusion: Under the intervention of proanthocyanidin in rats with kidney stones, the level of oxidative stress in the kidney decreases, the renal function improves, and the number of crystals in the kidney decreases, which proves that proanthocyanidin has a positive effect on preventing kidney stones and protecting renal function in rats.