组合生物反应器处理甲苯有机废气的性能及微生物研究

Study on Performance and Microorganism of Toluene Treatment by Combined Bioreactor

采用组合生物反应器对甲苯有机废气进行处理,考察了单独膜生物反应器(MBfR)、单独生物滴滤器(BTF)和组合生物反应器(BTF-MBfR)的长期运行稳定性,以及气体浓度、停留时间、循环液喷淋量、pH对反应器净化甲苯的影响;采用16S r RNA技术对组合反应器中的微生物群落结构进行了分析。结果表明,组合反应器实现100天稳定运行,甲苯的平均去除效率为94.9%,去除能力为41.02 g·m^(-3)·h^(-1);适宜的运行条件为停留时间10 s,pH为7.20,循环液喷淋量1.6~1.8 m^(3)·m^(-2)·h^(-1);组合反应器能够适应进气浓度更高的甲苯,在抗冲击负荷能力方面组合反应器优于单独的MBfR和BTF装置;16 S rDNA技术分析结果表明组合反应器中丰度≥1%的菌属有8种,其中假单胞菌属(Pseudomonas)和噬氢菌属(Hydrogenophaga)是组合反应器中的降解甲苯的主导菌属,丰度分别约为5.9%和3%。

Combined bioreactor, which was combined with biotrickling filter(BTF) and membrane biofilm reactor(MBfR),was used for the treatment of toluene gas. In this study, long-term stable operation of MBfR、BTF and BTF-MBfR were investigated. Influences of factors on toluene removal efficiency were also studied.Combined bioreactor achieved stable operation for 100 days, the average removal efficiency of toluene was up to 94.9 % and removal capacity was 41.02 g·m^(-3)·h^(-1).Suitable operating parameters were: circulating liquid spray density of 1.0~1.4 m^(3)·m^(-2)·h^(-1), circulating fluid p H value of 7.20~7.80 and the residence time of 10 s. The optimum operating conditions was: residence time of 10 s, pH=7.20, circulating liquid spray density of 1.6~1.8 m^(3)·m^(-2)·h^(-1). The combined bioreactor was able to withstand higher inlet concentration, and in the ability to resist impact load, combination bioreactor was superior to single MBfR and BTF. The results of 16 S r RNA showed that there were 8 bacteria(abundance ratio≥1 %) in the combination bioreactor. Among the 8 bacteria, Pseudomonas and Hydrogenophaga were the two dominant bacteria for toluene treatment, which abundance ratio were about 5.9 % and 3 %, respectively.