扩散峰度成像技术检测急性期癫痫大鼠模型的成像改变

A Study on Diffusion Kurtosis Imaging in Acute Epilepsy Rats

目的探讨扩散峰度成像(diffusion kurtosis imaging,DKI)技术对急性期癫痫大鼠的研究价值。方法Li-Pilocarpine诱导急性期大鼠癫痫模型13只,作为模型组,选取9只正常大鼠作为对照组。采用DKI技术对2组研究对象进行扫描,测量双侧海马、双侧梨状皮层/内嗅皮层的部分各向异性(fractional anisotropy,FA)、平均扩散系数(mean diffusivity,MD)、平均峰度(mean kurtosis,MK)、平行峰度(axial kurtosis,K∥)、径向峰度(radial kurtosis,K⊥)。采用SPSS 17.0统计学软件进行统计学分析。结果与对照组相比,模型组大鼠双侧梨状皮层/内嗅皮层的MD降低,MK、K∥、K⊥升高,差异具有统计学意义(P<0.05)。模型组大鼠双侧海马FA、MD、MK、K∥、K⊥的改变,差异无统计学意义(P>0.05)。结论DKI技术可以反映急性癫痫大鼠的微观病理改变,为急性期癫痫大鼠脑组织的病理生理改变提供理论依据。

Objective To investigate brain abnormalities in rats with acute epilepsy by diffusional kurtosis imaging(DKI).Methods MRI were performed on all rats.DKI images were obtained from 13 epilepsy rats and 9 normal controls.Fractional anisotropy(FA),mean diffusivity(MD),and mean kurtosis(MK),axial kurtosis(K∥),radial kurtosis(K⊥)of bilateral hippocampus,piriform cortex and entorhinal cortex were measured.SPSS 17.0 was used for statistical analysis.Results Compared with the control group,MD of bilateral piriform cortex/entorhinal cortex decreased,MK、K∥、K⊥significantly increased in the model group(P<0.05).There were no statistically significant differences in FA,MD,MK,K∥and K⊥in the bilateral hippocampus of model group.Conclusion Using DKI to reveal the pathologic changes of rats with acute epilepsy provide theoretical basis for the pathophysiological changes of brain tissues of rats with acute epilepsy.