摘要
目的探讨长链非编码RNA(Long non-coding RNA,IncRNA)APTR、HEIH、FAS-ASA1、FAM83HAS1、DICER1-AS1、PR-lncRNA在肺癌患者中的表达。方法收集昆明医科大学第三附属医院肺癌手术患者的癌组织,癌旁正常组织及肺癌淋巴结转移组织,利用TRIzol提取总RNA,再用SYBR Green实时荧光定量PCR检测每种基因的RNA相对表达量。结果APTR在淋巴组织中表达升高(P<0.05),HEIH在淋巴组织中表达下降(P<0.05),FAM83H-AS1和、PR-lncRNA在肺癌组织中表达升高(P<0.05)。结论APTR在淋巴组织中高表达,可能有促进肺癌淋巴结转移的作用;FAM83H-AS1及PR-lncRNA癌组织中表达上调,可能会促进肺癌的发生发展。
Objective To investigate the expression of long non-coding RNA(IncRNA)APTR,HEIH,FAS-ASA1,FAM83H-AS1,DICER1-AS1 and PR-lncRNA in lung cancer patients.METHODS Total RNA was extracted by TRI Z O L from cancer tissues,adjacent normal tissues and lymph node metastases of lung cancer patients in the Third Affiliated Hospital of Kunming Medical University.The relative RNA expression of each gene was detected by SYBR GR e e N real-time quantitative fluorescence PCR.RESULTS The expression of APTR was increased in lymphoid tissues(P<0.05),the expression of HEIH was decreased in lymphoid tissues(P<0.05),and the expression of FAM83H-AS1 and PR-lncRNA were increased in lung cancer tissues(P<0.05).Conclusion Increased expression of APTR in lymphoid tissue may promote lymph node metastasis of lung cancer;increased expression of FAM83H-AS1 and PR-lncRNA in cancer tissue may promote the occurrence and development of lung cancer.
作者
张腾飞
何越峰
张磊
张莉
杨凯云
刘莲清
谭慧
ZHANG Teng-fei;HE Yue-feng;ZHANG Lei;ZHANG Li;YANG Kai-yun;LIU Lian-qing;TAN Hui(Dept.of Thoracic Surgery,The 3rd Affiliated Hospital of Kunming Medical University,Kunming Yunnan,650118;School of Public Health,Kunming Medical University,Kunming Yunnan 650500,China)
出处
《昆明医科大学学报》
CAS
2021年第4期49-52,共4页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81860572)
云南省卫生科技计划基金资助项目(2016NS108)。
