NELFE通过抑制NDRG2表达促进三阴性乳腺癌细胞迁移和侵袭

NELFE promotes the migration and invasion of triple negative breast cancer cells by inhibiting NDRG2 expression

目的探讨负性延长因子E(negative elongation factor E,NELFE)对三阴性乳腺癌(triple-negative breast cancer,TNBC)细胞侵袭和迁移的作用及其作用机制。方法qRT-PCR检测NELFE在TNBC组织和癌旁组织中的表达;利用慢病毒转染技术构建NELFE表达上调的MDA-MB-231细胞系;Transwell小室实验观察NELFE表达升高对MDA-MB-231细胞迁移和侵袭能力的影响;RNA稳定性试验检测NELFE对N-myc下游调节基因2(N-myc downstream regulated gene2,NDRG2)mRNA稳定性的影响;Western blotting检测NELFE对NDRG2蛋白的调控作用。结果NELFE在TNBC组织中表达升高;过表达NELFE后,MDA-MB-231细胞迁移和侵袭能力明显增强,NDRG2 mRNA稳定性降低,NDRG2蛋白表达下调;在NELFE过表达的MDA-MB-231细胞系中上调NDRG2表达时,细胞迁移、侵袭能力降低。结论NELFE通过降低NDRG2 mRNA稳定性抑制其表达,促进TNBC细胞的迁移和侵袭能力。

Objective To explore the effect of negative elongation factor E(NELFE)on the invasion and migration of triple-negative breast cancer(TNBC)cells and its mechanisms.Methods qRT-PCR was used to detect the expression of negative elongation factor E(NELFE)in triple-negative breast cancer(TNBC)tissues and adjacent tissues.NELFE expression was upregulated in MDA-MB-231 cell lines via lentivirus transfection.Transwell assay was used to observe the effect of elevated NELFE expression on the migration and invasion of MDA-MB-231 cells.The RNA stability assay was used to detect the influence of NELFE on the stability of the mRNA of N-myc downstream regulated gene2(NDRG2).Western blotting analysis was used to detect the regulatory function of NELFE on NDRG2 protein expression.Results NELFE expression was increased in TNBC tissues.After the overexpression of NELFE,the migration and invasion of MDA-MB-231 cells were significantly enhanced,NDRG2 mRNA stability was decreased,and NDRG2 protein expression level was downregulated.NDRG2 expression was upregulated in MDA-MB-231 cell lines with overexpressed NELFE;cell migration and invasion abilities were decreased.Conclusion NELFE inhibited NDRG2 expression by decreasing the stability of NDRG2 mRNA and promoted the migration and invasion of TNBC cells.