基于SRAP分子标记的特早熟荔枝种质资源遗传多样性分析

Genetic Diversity Analysis of Very Early Maturing Litchi Germplasm Resources Based on SRAP Molecular Markers

以22份特早熟、6份早熟及2份中熟荔枝种质资源为材料,利用SRAP分子标记进行遗传多样性分析,探索特早熟荔枝种质资源的亲缘关系。结果表明:对182对SRAP引物组合进行筛选,获得16对多态性高、条带清晰的SRAP引物组合,共计产生扩增条带182条,其中多态性条带140条,占76.9%。材料间遗传相似系数为0.47~0.99,根据UPGMA聚类分析,在遗传相似系数0.73处可将30份荔枝样本分成4类,其中第Ⅰ类20份,占66.7%,包括绝大多数特早熟荔枝资源;第Ⅱ类4份,占13.3%,包括‘妃子笑’与部分杂交早熟资源;第Ⅲ类和第Ⅳ类各3份,分别占10%。以上研究结果为特早熟荔枝种质资源发掘、评价及新品种培育提供理论参考。

Utilizing the SRAP molecular markers technology,the genetic diversity and relationship of 30 litchi germplasm resources,including 22 extremely early maturity varieties,6 early maturity varieties and 2 middle maturity varieties,were analyzed in this study.The results showed that 16 pairs SPAP primers with high polymorphism and clear bands were selected in 182 pairs SPAP primers.A total of 182 loci were obtained using 16 pairs SPAP primers and 140 loci were polymorphic.UPGMA cluster analysis and genetic similarity coefficient showed that the similarity coefficient of 30 litchi germplasm resources ranged from 0.47 to 0.99,and 30 resources could be grouped into four distinct families based on similarity of 0.73.There were 20(66.7%)resources in groupⅠ,including the majority of extremely early maturing resources,4(13.3%)resources in groupⅡ,including’Feizixiao’and some hybrids,3(10.0%)resources respectively in groupⅢand groupⅣ.The results would provide theoretical references for the exploration,evaluation and new variety cultivation of extra-early litchi germplasm resources.