研究PODXL在肾透明细胞癌中的表达及相关意义

Exploration of the Expression and Significance of PODXL in Renal Clear Cell Carcinoma

目的:研究足细胞标志蛋白在透明细胞肾细胞癌组织与正常组织中的表达差异,并初步分析足细胞标志蛋白与肾透明细胞癌的临床诊断和患者预后的关系。方法:运用癌症基因组图谱数据库收集转录组测序技术数据和临床数据,观察透明细胞肾细胞癌患者组织与正常组织中足细胞标志蛋白基因表达量的差异,同时分析足细胞标志蛋白与临床病理学及预后之间的关系。结果:在癌症基因组图谱数据库中,总体526例透明细胞肾细胞癌样本的癌和癌旁组织中的足细胞标志蛋白的表达水平分别为(6.42±1.42)和(3.61±1.31),差异有统计学意义(P<0.001)。72例配对的透明细胞肾细胞癌和癌旁组织中的足细胞标志蛋白表达水平分别为(6.55±1.23)和(3.53±1.35),差异有统计学意义(P<0.001)。足细胞标志蛋白的表达因患者性别、病理分级、TNM分期、远处转移、临床分期等不同均有差异(P<0.001)。生存分析发现,足细胞标志蛋白低表达量组和高表达量组的生存时间分别为(1446.75±956.32)d和(1253.81±939.57)d,差异有统计学意义(P<0.001)。受试者工作特征曲线结果显示,足细胞标志蛋白基因可作为一个灵敏度和特异度较高的透明细胞肾细胞癌诊断指标:其中ROC曲线下面积为0.805,敏感性为67.26%,特异性为78.17%。结论:足细胞标志蛋白在透明细胞肾细胞癌中高表达与临床病理特征呈正相关,是预后不良因素,有望作为肾透明细胞癌患者的生存预后及诊断的潜在生物学标志。

Objective:To study the difference of expression of Podocalyxin-like Protein in clear cell renal cell carcinoma tissues and normal tissues,and to preliminarily analyze the relationship between the clinical diagnosis and prognosis of Podocalyxin-like Protein in clear cell renal cell carcinoma.Methods:Collected RNA-seq data and clinical data from the Cancer Genome Atlas database,in order to observe the difference of expression of Podocalyxin-like Protein in clear cell renal cell carcinoma tissues and normal tissues,and analyzed the relationship between the clinical pathology and prognosis of Podocalyxin-like Protein.Results:In the Cancer Genome Atlas database,the expression levels of Podocalyxin-like Protein in the carcinoma and paracancer tissues of 526 clear cell renal cell carcinoma samples were(6.42±1.42)and(3.61±1.31)respectively,with statistically significant differences(P<0.001).The expression levels of Podocalyxin-like Protein in 72 paired clear cell renal cell carcinoma and paracancer tissues were(6.55±1.23)and(3.53±1.35)respectively,with statistically significant differences(P<0.001).The expression of Podocalyxin-like Protein varies with the patient's gender,pathological grade,TNM stage,distant metastasis and clinical stage(P<0.001).Survival analysis showed that the survival time of Podocalyxin-like Protein in low-expression group and high-expression group were(1446.75±956.32)d and(1253.81±939.57)d respectively,with statistically significant differences(Log-rank=14.38,P<0.001).The results of receiver operating characteristic curve showed that Podocalyxin-like Protein gene could be used as a diagnostic index with high sensitivity and specificity for clear cell renal cell carcinoma:the area under the ROC curve was 0.805,the sensitivity was 67.26%,and the specificity was 78.17%.Conclusion:The high expression of Podocalyxin-like Protein in clear cell renal cell carcinoma is positively correlated with clinicopathological features,and is a poor prognostic factor,which is expected to be a potential biological marker for survival prognosis and diagnosis in patients with clear cell carcinoma.