基于NMR的高脂血症小鼠血浆代谢组学研究

NMR-based metabolomics of hyperlipidemia mice plasma

运用1H NMR技术研究高脂血症小鼠血浆代谢组学特征,阐明高脂血症病理机制。将90只昆明小鼠随机分为18组,9个对照组和9个高脂组,在第7 d、10 d、15 d、18 d、21 d、24 d、28 d、31d和35 d取血浆进行1H NMR全谱(NOESY)分析,采用主成分分析及正交偏最小二乘判别分析法研究高脂组和对照组之间的代谢谱差异,筛选潜在生物标志物。结果表明,高脂组与对照组相比,血浆缬氨酸、乳酸、丙氨酸、谷氨酸、谷氨酰胺、柠檬酸、二甲基甘氨酸、酪氨酸、组氨酸、牛磺酸、青蟹肌醇、延胡索酸、苯丙氨酸、尿嘧啶差异有统计学意义(P<0.05)。这些代谢物显著影响了苯丙氨酸、酪氨酸和色氨酸的生物合成;显著影响了谷氨酰胺和谷氨酸代谢,苯丙氨酸代谢,组氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢等代谢途径,参与了高脂血症的病理生理过程。

To elucidate hyperlipidemia metabolomics characteristics and mechanism based on fingerprint of 1H NMR.90 Kunming male mice were randomly divided into 18 groups,the control group and high fat diet groups.Blood was collected on days 7,10,15,18,21,24,28,31,35 for 1H NMR analysis.Potential biomarkers were screened by principal component analysis and orthogonal partial least squares discriminant analysis to study the differences in metabolic profiles between the high-fat group and the control group.The plasma valine,lactate,alanine,glutamine,glutamine,citrate,dimethyl glycine,tyrosine,histidine,taurine,scyllo-inositol,fumarate,phenylalanine and uracil were significantly different between the high fat diet groups and the corresponding control groups(P<0.05).These significantly affected metabolic pathways,namely phenylalanine,tyrosine and tryptophan biosynthesis,glutamine and glutamate metabolism,phenylalanine metabolism,histidine metabolism,alanine,aspartate and glutamate metabolism,and were involved in the pathophysiological process of hyperlipidemia.