羊骨胶原肽钙螯合物对去卵巢大鼠肾组织病变的改善作用

Sheep bone collagen peptide calcium chelate ameliorates renal tissue lesions in ovariectomized rats

目的探究羊骨胶原肽钙螯合物(collagen peptide chelated calcium,CPCC)对雌激素缺乏大鼠肾的保护作用及相关机制。方法摘除大鼠双侧卵巢,CPCC高(CPCC-H)和低(CPCC-L)剂量组的灌胃剂量分别为5 g/(kg·d)和1 g/(kg·d),假手术组和模型组灌胃等体积蒸馏水。8周后测定肾抗氧化指标并观察肾组织形态学变化;qRT-PCR测定JAK/STAT通路相关基因mRNA表达量。结果与假手术组相比,去卵巢模型组大鼠肾TSOD、GSH-Px和T-AOC水平极显著下降、MDA含量极显著增加(P<0.01),肾组织结构紊乱、淋巴细胞浸润;血清中炎性因子IL-2、TNF-α和IFN-γ水平极显著增加(P<0.01),JAK2、STAT1和STAT3基因mRNA的相对表达量极显著增加(P<0.01)。与模型组相比,CPCC剂量组的T-SOD、GSH-Px和T-AOC水平极显著增加,MDA含量极显著下降(P<0.01);肾组织病变明显减轻,淋巴细胞数量减少;血清中各炎性因子水平极显著下降(P<0.01);JAK2、STAT1和STAT3基因mRNA表达量均极显著下降(P<0.01)。结论CPCC可抑制去卵巢大鼠的氧化应激水平,可能通过JAK/STAT通路改善肾组织病变。

Objective To investigate the protective effect and the underlying mechanisms of action of sheep bone collagen peptide chelated calcium(CPCC)on the kidney of estrogen-deficient rats.Methods Rats were subjected to bilateral ovariectomy.The CPCC dosages for the high-dose(CPCC-H)and low-dose(CPCC-L)groups were 5 g/(kg·d)and 1 g/(kg·d),respectively.In the model and sham groups,the same volume of distilled water was intragastrically administered every day.After 8 weeks,the kidney antioxidant indexes were measured,and the morphological changes in kidney tissues were observed.qRT-PCR was used to measure the mRNA expression of JAK/STAT signaling pathway-related genes.Results Compared with the sham group,the kidney T-SOD,GSH-Px and T-AOC activities in the model group were decreased significantly,and the MDA content was increased significantly(P<0.01).Furthermore,normal renal tissue structure was perturbed and lymphocyte infiltration was observed.Levels of the serum inflammatory factors IL-2,TNF-α and IFN-γ were increased significantly(P<0.01).qRT-PCR analysis showed that the mRNA expression levels of the JAK2,STAT1 and STAT3 genes were increased significantly(P<0.01).After CPCC treatment,compared with the model group,T-SOD,GSH-Px and T-AOC levels increased significantly(P<0.01),and MDA content decreased significantly(P<0.01).Renal tissue lesions were significantly reduced,and the number of lymphocytes was decreased.Furthermore,serum levels of various inflammatory factors were decreased significantly(P<0.01),and mRNA expression levels of JAK2,STAT1 and STAT3 genes were decreased significantly(P<0.01).Conclusions CPCC inhibits oxidative stress in ovariectomized rats and may ameliorate renal tissue pathology through the JAK/STAT signaling pathway.