3种生长调节剂对马铃薯试管苗生长的影响

Effects of Three Growth Regulators on the Growth of Potato Test-Tube Seedlings

为提高试管苗品质,优化马铃薯脱毒继代培养,以延薯4号脱毒苗为试验材料,选用6-BA(0.5、1.0、1.5mg/L)、水杨酸(0.2、0.4、0.6mg/L)和油菜素内酯(0.01、0.10、1.00mg/L)处理试管苗,以不添加生长调节剂为对照,研究3种生长调节剂对马铃薯试管苗生长形态特征、叶绿素含量、根活力、移栽后幼苗成活率及结薯特性的影响,结果显示,3种生长调节剂均能在一定程度上增加株高、茎粗、鲜重、平均根长、根条数和叶片数,6-BA、水杨酸和油菜素内酯分别在1.0mg/L、0.4mg/L和1mg/L处理时促进效果较好,叶绿素含量、根活力以及移栽后幼苗成活率和结薯特性均较好,且在水杨酸浓度为0.4mg/L时,试管苗生长最好,移栽后成活率最高,结薯特性较好,是最适壮苗培养基。

To improve the quality of test-tube seedlings and optimize the virus-free subculture of potato, Yanshu No. 4 virus-free seedlings were used as the test material, and 6-BA(0.5 mg/L, 1.0 mg/L, 1.5 mg/L), salicylic acid(0.2 mg/L,0.4 mg/L, 0.6 mg/L) and brassinolide(0.01 mg/L, 0.10 mg/L, 1.00 mg/L) was selected as the treatments, while, no growth regulator was added in control. The growth morphological characteristics, chlorophyll content, root vitality, survival rate and tuber formation characteristics of three growth regulators on potato test-tube seedlings were studied. The results showed the three growth regulators could increase plant height, stem diameter, fresh weight, average root length, root strip number and leaf number. In addition, the chlorophyll content, root vitality, survival rate and tuber formation characteristics after transplanting were all better under the treatment of 6-BA, salicylic acid and brassinolide are 1.0 mg/L,0.4 mg/L and 1.0 mg/L, respectively. After transplanting, the survival rate was the highest, and the tuber formation characteristics were better under the treatment of salicylic acid(0.4 mg/L), which is the most suitable medium for strong seedlings.