病毒性心肌炎小儿外周血FOXP3 mRNA、GARP mRNA、miR98的变化与预后的关系研究

Relationships Between Changes of FOXP3 mRNA,GARP mRNA,miR98 in Peripheral Blood and Prognosis for Viral Myocarditis Children

目的探讨病毒性心肌炎小儿外周血FOXP3 mRNA、GARP mRNA、miR98水平及与预后的关系。方法选取2018年1月至2019年4月在我院治疗的病毒性心肌炎患儿97例(观察组),根据左心室射血分数(LVEF)水平,轻症患儿62例,重度患儿35例;同时选取健康体检儿童90例作为对照组。检测外周血FOXP3 mRNA、GARP mRNA、miR98水平。结果观察组外周血FOXP3 mRNA、GARP mRNA、miR98相对表达量分别为(0.32±0.09)、(0.41±0.12)和(0.60±0.10),低于对照组(1.01±0.20)、(1.08±0.22)、(1.12±0.14),差异有统计学意义(P<0.05);观察组重症患儿外周血FOXP3 mRNA、GARP mRNA、miR98相对表达量低于轻症患儿(P<0.05);观察组死亡患儿外周血FOXP3 mRNA、GARP mRNA、miR98相对表达量低于存活患儿(P<0.05);FOXP3 mRNA、GARP mRNA、miR98相对表达量与LVEF呈负相关(r=-0.464、-0.366和-0.311,P<0.05)。结论病毒性心肌炎小儿外周血FOXP3 mRNA、GARP mRNA、miR98降低,与患儿病情程度及预后有一定关系。

Objective To investigate FOXP3 mRNA,GARP mRNA and miR98 in peripheral blood of viral myocarditis children and their relationship with prognosis.Methods From January 2018 to April 2019,97 children with viral myocarditis treated in our hospitalwere selected as observation group.According to left ventricular ejection fraction(LVEF),62 children were mild disease and 35 children were severe disease.At the same time,90 healthy children were selected as control group.FOXP3 mRNA,GARP mRNA and miR98 in peripheral blood were detected. Results In observation group,relative expressions of FOXP3 mRNA,GARP mRNA and miR98 in peripheral blood were(0. 32±0. 09),(0. 41±0. 12) and(0. 60±0. 10),respectively,which were lower than those of control group that were(1. 01±0. 20),(1. 08±0. 22) and(1. 12±0. 14),respectively,with statistically significant differences(P<0. 05).Meanwhile,relative expressions of FOXP3 mRNA,GARP mRNA and miR98 in peripheral blood of severe children were lower than those in mild children(P< 0. 05). The relative expressions of FOXP3 mRNA,GARP mRNA,and miR98 in peripheral blood of died children were lower than those of surviving children(P < 0. 05). The relative expressions of FOXP3 mRNA,GARP mRNA,and miR98 were negatively correlated with LVEF(r =-0. 464,-0. 366 and-0. 311,P< 0. 05).Conclusion For viral myocarditis children,FOXP3 mRNA,GARP mRNA,and miR98 in peripheral blood would decrease,that has a certain relationship with severity and prognosis.