白菜CRM基因家族的生物信息学分析

Bioinformatics analysis of CRM gene family in Brassica rapa

CRM(Chloroplast RNA splicing and ribosome maturation)蛋白结构域参与调控植物线粒体和叶绿体内包含Ⅱ型内含子基因的剪接。研究利用生物信息学的方法,对白菜(Brassica rapa,AA,2n=20)CRM基因家族的基因数量、保守基序、进化关系、氨基酸等电点以及表达量差异等进行分析。结果表明,白菜CRM基因家族共包含22个基因,可分为3个亚族,分布于白菜除A10外的9条染色体上。CRM蛋白编码基因的长度在657~5909 bp,蛋白质等电点为4.42~9.89,大多数的蛋白质偏碱性。在表达模式上,不同组织和不同胁迫下白菜CRM基因的表达量存在较大差异,部分CRM基因在叶和茎中的转录表达较活跃;以Flg22和Ps模拟生物胁迫,部分CRM基因的表达量显著下调,CRM基因可能响应植物的生物胁迫。

The CRM(Chloroplast RNA splicing and ribosome maturation)genes involve in the splicing of genes containing groupⅡintrons in mitochondria and chloroplast genome in plants.In the present study,gene number,conserved motif,isoelectric point of amino acids,molecular evolution,and expression pattern of CRM family of Brassica rapa(AA,2n=20)were well analyzed based on the bioinformatic methods.A total of 22 genes belonging to CRM gene family were identified in the B.rapa genome.These genes could be divided into three subgroups,and distributed on all the chromosomes of B.rapa except chromosome A10.The coding sequences of CRM genes ranged from 657 bp to 5909 bp,and the isoelectric point of amino acids of these genes ranged from 4.42 to 9.89.The CRM genes in B.rapa showed specific expression in different tissues and response to different stresses,and some CRM genes were actively transcribed in leaves and stems.After dealt with Flg22 and Ps stress which can be used to simulate biotic stresses,some CRM genes significantly down-regulated,indicating these genes likely responded to the biotic stresses.