miR-133b、miR-495在骨肉瘤组织中表达的临床意义及相关性分析

Clinical implications and correlation analysis of MiR-133b and MiR-495 expression in osteosarcoma tissues

目的:探讨miR-133b、miR-495在骨肉瘤组织中的表达特点,分析其与骨肉瘤临床病理参数和预后的关系。方法:选择79例经手术切除的骨肉瘤组织标本(骨肉瘤组)以及瘤旁组织标本(对照组,距离瘤组织>5 cm),采用实时PCR法检测组织中miR-133b、miR-495表达。收集患者临床病理参数相关信息,术后定期随访,比较不同临床病理参数患者miR-133b、miR-495表达差异,分析miR-133b、miR-495与骨肉瘤患者预后的关系。结果:骨肉瘤组miR-133b、miR-495表达低于正常组(P<0.05),Enneking分期Ⅲ期、淋巴结转移、远处转移患者骨肉瘤组织中miR-133b、miR-495表达低于Enneking分期Ⅰ期、Ⅱ期、未发生淋巴结转移和远处转移患者(P<0.05)。Kaplan-Meier生存分析结果显示低miR-133b表达组、低miR-495表达组骨肉瘤患者生存率低于高miR-133b表达组、高miR-495表达组(P<0.05)。多因素Cox风险比例回归分析结果显示淋巴转移、远处转移、低表达miR-133b、miR-495是骨肉瘤患者死亡的危险因素(P<0.001)。结论:骨肉瘤组织中miR-133b、miR-495表达均降低,miR-133b、miR-495低表达可能参与骨肉瘤恶性进展过程及患者不良预后有关。

Objective:To investigate the expression features of miR-133b and miR-495 in osteosarcoma tissues,and analyze their relationship with the clinicopathological parameters and prognosis of osteosarcoma.Methods:Tissue samples were obtained from 79 patients with osteosarcoma undergone surgical resection in our department,and divided into osteosarcoma group and control group(adjacent tissues taken at>5 cm distant from the tumor),and clinicopathological parameters were collected in all patients.Real-time PCR was used to detect miR-133b and miR-495 expression in the cancerous and adjacent tissues.Then the two groups were compared regarding the difference in clinicopathological parameters and miR-133b and miR-495 expression for estimation of the prognosis on regular follow-up basis.Results:Expression of miR-133b and miR-495 was lower in osteosarcoma group than in control group(P<0.05),and also lower in patients of Enneking stageⅢ,with lymph node and distant metastasis than in those of Enneking stageⅠorⅡ,without lymph node and distant metastasis(P<0.05).Kaplan-Meier survival analysis showed that the survival rate was lower in osteosarcoma patients with low miR-133b and miR-495 expression than in those with higher miR-133b and miR-495 expression(P<0.05).Multivariate Cox proportional-hazard regression analysis revealed that lymphatic metastasis,distant metastasis,low expression of miR-495 and miR-133b were risk factors for death in patients with osteosarcoma(P<0.001).Conclusion:Expression of miR-133b and miR-495 is decreased in osteosarcoma tissues,suggesting that the two genes are possibly involved in progression of this malignancy,and related to poor prognosis of such patients.