miR-200a以及上皮性卵巢癌病理特征、预后的关系研究

Relationship between miR-200a and pathological features and prognosis of epithelial ovarian cancer

目的探讨卵巢癌组织中微小RNA-200a(miR-200a)在上皮性卵巢癌组织中的表达与病理学特征及预后的关系。方法选取在本院病理科经病理学检查证实的卵巢癌组织标本120例(恶性组)、120例卵巢良性肿瘤组织标本(良性组);采用实时荧光聚合酶链反应(RT-PCR)技术检测两组标本中的miR-200a表达水平,分析不同年龄、FIGO分期、组织学分化、淋巴结转移、病理学类型、3年不同随访结局卵巢癌组织中miR-200a表达的差异;采用Logistic回归分析法探讨miR-200a与上皮性卵巢癌患者预后的关系。结果恶性组标本中的miR-200a相对表达水平显著高于良性组的黏液性囊腺瘤、浆液性囊腺瘤,差异具有统计学意义(P<0.05);FIGO分期(Ⅲ期+Ⅳ期)、低分化、发生淋巴结转移、3年随访死亡的卵巢癌组织标本中的miR-200a相对表达水平显著高于FIGO分期(Ⅰ期+Ⅱ期)、高中分化、未发生淋巴结转移、3年随访存活的患者,差异具有统计学意义(P<0.05);不同年龄分组、不同病理学类型的卵巢癌组织标本中的miR-200a相对表达水平差异无统计学意义(P>0.05);经Logistic回归分析,卵巢癌患者FIGO分期(Ⅲ期+Ⅳ期)、低分化、发生淋巴结转移、miR-200a相对表达高水平是上皮性卵巢癌不良预后的独立危险因素(P<0.05)。结论miR-200a相对表达高水平可能会增加上皮性卵巢癌不良预后的风险。

Objective To investigate the relationship between microRNA-200a(miR-200a)expression in ovarian cancer tissues and pathological features and prognosis in epithelial ovarian cancer.Methods 120 cases of ovarian cancer tissue specimens confirmed by pathology in our hospital(malignant group)and 120 ovarian benign tumor tissue specimens(benign group)were selected.Real-time fluorescence polymerase chain reaction(RT-PCR)was used to detect the expression level of miR-200 a in the two groups.The differences of miR-200a expression in ovarian cancer tissues at different ages,FIGO stage,histological differentiation,lymph node metastasis,pathological type,and 3-year follow-up were analyzed.Logistic regression analysis was used to investigate the expression of miR-200a in different ages.The relationship between miR-200a and prognosis in patients with epithelial ovarian cancer.Results The relative expression level of miR-200a in the malignant group was significantly higher than that in the benign group,and the difference was statistically significant(P<0.05).FIGO stage(stage Ⅲ+Ⅳ),poor differentiation,lymph node metastasis,and 3-year follow-up death.The relative expression level of miR-200a in ovarian cancer tissue samples was significantly higher than that in FIGO stage(stage Ⅰ+Ⅱ),high-segment differentiation,no lymph node metastasis,and 3-year follow-up survival.The difference was statistically significant(P<0.05).There was no significant difference in the relative expression level of miR-200a in ovarian cancer tissues with different age groups and different pathological types(P>0.05).Logistic regression analysis showed FIGO stage of ovarian cancer patients(stage Ⅲ+Ⅳ),poor differentiation,lymph node metastasis,and high relative expression of miR-200a were independent risk factors for poor prognosis of epithelial ovarian cancer(P<0.05).Conclusion Relatively high levels of miR-200a expression may increase the risk of poor prognosis in epithelial ovarian cancer.