陆地棉转录因子GhAHL4基因的克隆及表达分析

Cloning and Expression Analysis of GhAHL4 Gene in Gossypium hirsutum

含有AT-hook基序的AHL转录因子不仅能够调控植物生长发育及器官建成,也在植物响应逆境胁迫和激素信号应答中起重要作用。为解析AHL在陆地棉中的结构和功能,基于已公布的陆地棉转录组数据挖掘AHL基因,并以冀丰1271为材料克隆了GhAHL4基因的编码序列,利用生物信息学工具和RT-qPCR进行结构和差异表达模式分析。结果表明,GhAHL4基因位于D11号染色体上,开放阅读框为984 bp,编码327个氨基酸,为定位于细胞核中的碱性亲水性蛋白;基因含有5个外显子和4个内含子,启动子含有ABRE、G-Box和LTR等非生物胁迫响应元件;GhAHL4含有一个AT-hook基序和一个PPC结构域,与亚洲棉GaAHL4亲缘关系最近;RT-qPCR揭示,GhAHL4基因的表达受盐胁迫诱导。研究结果可为深入解析棉花GhAHL家族成员的生物学功能以及培育高抗逆性棉花新种质提供科学参考。

The AHL transcription factors containing AT-hook motif not only regulate plant growth and development and organ formation,but also play an important role in regulation of stress response and hormonal signaling.To explore the structure and function of AHL in cotton,AHL4 gene of Gossypium hirsutum was identified based on transcriptome data.GhAHL4 gene was cloned in Jifeng 1271,a cultivar of Gossypium hirsutum,and its structure and differential expression pattern were analyzed by bioinformation tools and RT-qPCR.The results showed that the GhAHL4 gene was located on chromosome D11,and the open reading frame of GhAHL4 was 984 bp,encoding 327 amino acids,which was alkaline,hydrophilic protein located in the nucleus.The gene contained 5 exons and 4 introns,and its promoter contained abiotic stress response elements such as ABRE,G-Box and LTR.GhAHL4 contained an AT-hook motif and a PPC domain,which was closely related to GaAHL4.RT-qPCR analysis showed that expression of GhAHL4 was induced by NaCl stress.This study results will provide a scientific reference for further research on the biological functions of members of GhAHL family and the cultivation of new cotton germplasm with high stress resistance.