不同刮痧力度对大鼠皮肤形态及5-羟色胺、肥大细胞表达变化的比较研究

A Comparative Study on The Changes of Rat Skin Morphology,5-hydroxy-tryptamine and Mast Cell Expression with Different Gua Sha Intensity

目的:通过刮痧后实验大鼠皮肤形态及化学物质表达的变化,探究力度对刮痧产生的影响。方法:将健康SD大鼠随机分为空白组、轻刮痧组、中刮痧组、重刮痧组每组各6只,空白组常规饲养,轻刮痧组使用0.4kgf力,中刮痧组使用0.8kgf力,重刮痧组使用1.2kgf力,对其右侧阳陵泉穴进行刮痧干预均刮拭20次。干预1次后即刻取各组大鼠右侧穴区皮肤组织(10mm×15mm),之后运用荧光免疫组化技术进行肥大细胞(Mast Cell,MC)和5-羟色胺(5-hydroxytryptamine,5-HT)的检测。结果:与空白组比较,轻刮痧后皮肤组织形态无明显变化,5-HT在真皮层和皮下组织中沿毛囊与血管形成连续的增强带,MC表达增加;中刮痧后表皮层结构排列紊乱,MC、5-HT表达稍减少;重刮痧后皮肤组织形态明显紊乱,5-HT、MC明显较少。通过对阳性细胞进行计数,观察到轻刮痧组5-HT和MC的阳性标记数明显高于空白组,中刮痧组呈下降趋势,而重刮痧组则明显少于空白组。结论:一定的刮痧力度对于皮肤是安全的,适度刮痧会引起局部皮肤化学物质的表达响应变化,但是不会因为刮痧力度的增加而增强。

Objective:To explore the effects of intensity on Guasha through the changes of skin morphology and chemical substance expression in experimental rats after Guasha.Methods:Healthy SD rats were randomly divided into the blank group,light Guasha group,middle Guasha group,and heavy Guasha group,with 6 rats in each group.The blank group was routinely reared,0.4 kgf force was used in the light Guasha group,0.8 kgf force was used in the middle Guasha group,and 1.2 kgf force was used in the heavy Guasha group,and Guasha interventions were performed on theYanglingquan point on the right side.Immediately after Guasha,skin tissues(10mm×15mm)were taken from the right acupoint area of each group of rats,and fluorescent immunohistochemical technique were used to detect mast cell(MC)and 5-hydroxytryptamine(5-HT)antibodies.Results:Compared with the blank group,the skin morphology did not change significantly after light Guasha.5-HT formed continuous enhancement bands along with the hair follicles and vessels in the dermis and subcutaneous tissues,and the expression of MC increased.The expressions of MC and 5-HT were slightly reduced;the morphology of skin tissue was obviously disordered after heavy Guasha,and 5-HT and MC were significantly less.By counting positive cells,it was observed that the light Guasha group was significantly higher than the blank group,the middle Guasha group showed a downward trend,and the heavy Guasha group was significantly less than the blank group.Conclusion:Certain Guasha strength is safe for the skin.Moderate Guasha will cause changes in the expression response of local skin chemicals,and it will not be more significant with increasing Guasha strength.