雄性Kunming-DY大鼠血尿酸水平的主要影响因素

Main Factors Influencing Male Kunming-DY Rats’Serum Uric Acid Level

目的研究正常饲养条件下,影响雄性尿酸酶缺失大鼠(Kunming-DY大鼠)血尿酸水平的主要因素。方法取日龄为45 d的雄性Kunming-DY大鼠、杂合子大鼠和野生型大鼠,断尾取血制备血清,测定血清尿酸、血尿素氮和血肌酐水平。将大鼠置于代谢笼内监测其24 h摄入量,收集24 h的排泄物,检测排泄物的尿酸含量。对比分析各组大鼠的检测结果,同时对Kunming-DY大鼠组内的各因素进行相关性分析。结果与野生型大鼠或杂合子大鼠相比,Kunming-DY大鼠体质量明显偏轻,饮水量增加,血尿酸、血尿素氮和血肌酐值升高,尿量增多、尿液和粪便尿酸排泄量增加,血尿酸水平与进食量呈负相关。结论45日龄雄性Kunming-DY大鼠血尿酸水平主要由内源性尿酸生成增多引起,尿酸酶缺失对生长和肾功能存在不利影响。

Objective To study the main factors that influence the serum uric acid in male uricase-deficient rats(Kunming-DY rats)under normal feeding condition.Methods Male Kunming-DY rats,heterozygote rats,and wild-type rats of 45 d old were used in the present study.Their blood without anticoagulation was drawn from their cut tails,and serum samples were obtained by centrifugation.The serum uric acid(SUA),blood urea nitrogen(BUN),and creatinine(Cr)were assayed.Then,the rats were put in metabolic cages to record their intakes and excretion in 24 h.Their urine and feces excreted were collected,and the uric acid was detected.The results between the 3 groups were analyzed,and the relationship between factors in Kunming-DY rats were correlated.Results Compared with the wild-type and heterozygote rats,Kunming-DY rats’growth was retarded,though they consumed more water.Their SUA,BUN,Cr,and urine increased,and uric acid in the urine and feces also increased.There was a negative relationship between SUA and food consumption.Conclusion The increased SUA in Kunming-DY rats is mainly caused by uric acid synthesized from endogenous purines,and the uricase-deficiency is a disadvantage factor on rats’growth and renal function.