胃癌PTBP3表达状态与中医证型的相关性研究

Relationship between PTBP3 expression status and traditional Chinese medicine syndromes in gastric cancer

目的:通过检测PTBP3在胃癌组织中的表达差异,分析PTBP3表达水平与胃癌脾气虚证的相关性,探讨胃癌脾气虚证的危险因素。方法:收集胃癌新发病例,根据中医症状调查表进行中医辨证分型,并收集手术或胃镜石蜡切片,对肿瘤组织进行免疫组化染色,采用Pearson相关性分析和Logistic回归分析以探讨PTBP3与胃癌脾气虚证的关系。结果:共有228例胃癌患者纳入本研究。其中,男性169例,女性59例;Ⅰ期患者28例,Ⅱ期36例,Ⅲ期126例,Ⅳ期38例;中医辨证分型为脾气虚证者109例,非脾气虚证者119例;PTBP3低表达者97例,高表达者131例。Pearson分析显示,PTBP3表达与脾气虚证具有一定的相关性(R2=0.166,P<0.01),多因素Logistic回归分析结果显示,PTBP3高表达和肿瘤家族史为脾气虚证的独立危险因素(P<0.05)。结论:PTBP3表达状态与胃癌脾气虚证相关;PTBP3高表达是胃癌脾气虚证的独立危险因素。

Objective:To explore the the risk factors of gastric cancer with spleen deficiency syndrome,the correlation between PTBP3 expression level and gastric cancer with spleen deficiency syndrome was analyzed via detecting PTBP3 expression difference in gastric cancer.Methods:Patients initially diagnosed with gastric cancer were collected.TCM syndrome differentiation was performed by TCM symptom questionnaire.The tumor tissues after operation or gastroscopy were obtained for immunohistochemical staining.Pearson correlation and Logistic regression were used to analyze the relationship between PTBP3 and gastric cancer with spleen deficiency syndrome.Results:A total of 228 cases(169 male patients and 59 female patients)of gastric cance were included in this study.The conditions of patients respectively are:stage I(28 cases),stage II(36 cases),III stage(126 cases),stage IV(38 cases);spleen-deficiency syndrome(109 cases),non spleen-deficiency syndrome(119 cases),low expression of PTBP3(97 cases),high expression of PTBP3(131 cases).Pearson analysis showed a certain correlation(R2=0.166,P<0.01)between PTBP3 expression and spleen deficiency syndrome.Multiple Logistic regression analysis showed that cancer family history and the high expresstion of PTBP3 were two independent risk factors from spleendeficiency syndrome(P<0.05).Conclusion:The expression status of PTBP3 is closely related to spleen deficiency syndrome in gastric cancer.High expression of PTBP3 is an independent risk factor for gastric cancer with spleen deficiency syndrome.