黄连总碱片对实热焦虑模型的抗焦虑作用及机制

Anti-anxiety effect and mechanism of total alkaloid tablets of Rhizoma Coptidis based on excess heat anxiety model

目的:基于大鼠实热证焦虑模型观察黄连总碱片的抗焦虑作用及机制。方法:将SD大鼠分为空白组,对照组,模型组,阳性组,黄连总碱片高、中、低剂量组,采用灌胃党参黄芪水煎液14d的方法制成实热证模型。阳性组灌胃地西泮混悬液,黄连总碱片高、中、低剂量组灌胃相应剂量治疗,连续5d。观察大鼠的一般形态、肛温,末次给药30min后,进行高架十字迷宫(EPM)和明暗箱实验,结束后立即取血、脑及右叶肝组织。结果:与模型组比较,黄连总碱片可显著升高实热焦虑大鼠在EPM中的开臂滞留时间百分比及开臂进入次数百分比;显著升高脑组织中γ-氨基丁酸(GABA)、5-羟色胺(5-HT)含量,降低谷氨酸(Glu)含量,显著降低血浆促肾上腺皮质激素、皮质酮、血清三碘甲状腺原氨酸、四碘甲状腺原氨酸、促甲状腺激素及肝组织中琥珀酸脱氢酶的含量,显著升高血清反碘甲状腺原氨酸含量。结论:黄连总碱片对实热焦虑模型具有抗焦虑作用,其作用机制可能与增强GABA、5-HT水平,抑制Glu水平与抑制下丘脑-垂体-肾上腺轴、下丘脑-垂体-甲状腺功能亢进,以及解热作用有关。

Objective:To observe the anti-anxiety effect and mechanism of total alkaloid tablets of Rhizoma Coptidis on the anxiety model of excess heat syndrome in rats.Methods:SD rats were divided into three groups:blank group,control group,model group,positive group,high,medium and low dose of total alkaloid tablets of Rhizoma Coptidis group(H,M,L group).The model of excess heat syndrome was established by the method of intragastric administration of Dangshen Huangqi Decoction for 14 days.The positive group was given Diazepam suspension,while H,M,L group were given corresponding doses of total alkaloid of Rhizoma Coptidis for 5 days.The general morphology and anal temperature of rats were observed.After the last administration for 30 min,the elevated cross maze(EPM)and light dark box(LDT)experiments were carried out.Then blood,brain and right lobe liver tissues were taken immediately.Results:Compared with the model group,the M group could significantly increase the OE%and OT%in the EPM test in rats with excess heat anxiety.And it significantly increased GABA,5-HT and decreased Glu in brain tissue,and decreased plasma ACTH,CORT and serum T3,T4,TSH and SDH in liver tissue,increased serum rT3.Conclusion:Total alkaloid tablets of Rhizoma Coptidis has anti-anxiety effect on excess heat anxiety model,and its mechanism may be related to the enhancement of GABA and 5-HT level,the inhibition of Glu level,hyperfunction of hypothalamic pituitary adrenal(HPA)axis and hypothalamic pituitary thyroid(HPT)axis,and its antipyretic effect.