硒化蒲公英多糖的制备、结构表征及益生菌促增殖活性

Preparation, Structural Characterization and Probiotics Proliferation-Promoting Activity of Selenized Dandelion Polysaccharide

本实验以蒲公英根为原料,采用硝酸-亚硒酸钠法制备硒化蒲公英多糖(selenizeddandelionroot polysaccharide,Se-DRP),研究其理化性质、结构、对益生菌增殖的促进作用和对α-淀粉酶的抑制活性。结果表明:Se-DRP是一种分子质量为8 102 Da的杂多糖,由半乳糖醛酸、鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖6种单糖组成,物质的量比为0.64∶0.73∶19.36∶0.84∶1.00∶27.41;核磁共振结果表明,组成Se-DRP的主要糖残基为→6-β-D-葡萄糖-(1→、→5)-α-L-阿拉伯糖-(1→、→3)-β-D-半乳糖-(1→和→4)-α-D-半乳糖醛酸-(1→;Se-DRP能促进益生菌的增殖,植物乳杆菌10665和嗜酸乳杆菌可将Se-DRP作为碳源利用;同时,Se-DRP对α-淀粉酶活性具有一定的抑制作用,在质量浓度为4.0 mg/mL时,抑制率为(52.34±1.45)%。以上结果为进一步研究Se-DRP及蒲公英资源的开发利用提供了一定的理论依据。

In this study,a selenized polysaccharide from dandelion roots(Se-DRP)was prepared by HNO3-Na2 SeO3 method.The physicochemical properties,structural characteristics,probiotics proliferation-promoting activity of Se-DRP and its inhibitory effect onα-amylase were investigated.The results showed that Se-DRP was a heteropolysaccharide with a molecular mass of 8102 Da,and it was composed of galacturonic acid,rhamnose,arabinose,mannose,glucose and galactose with a molar ratio of 0.64:0.73:19.36:0.84:1.00:27.41.Nuclear magnetic resonance(NMR)analysis showed that the major sugar residues in Se-DRP were→6)-β-D-glucose-(1→,→5)-α-L-arabinose-(1→,→3)-β-D-galactose-(1→and→4)-α-D-galacturonic acid-(1→.Se-DRP possessed probiotics proliferation-promoting activity and could be utilized by Lactobacillus plantarum 10665 and Lactobacillus acidophilus as a carbon source.In addition,Se-DRP exhibitedα-amylase inhibitory activity with an inhibitory rate of(52.34±1.45)%at a concentration of 4.0 mg/mL.These results provide a theoretical basis for further studies of Se-DRP and the utilization of dandelion resources.