肉桂精油对小鼠肝肠组织抗氧化能力以及肠道菌群结构的调节作用

Regulatory Effect of Cinnamon Essential Oil on Antioxidant Capacity of Liver and Intestine Tissues and Gut Microbiota in Mice

目的:探究肉桂精油对小鼠肝脏代谢相关酶活性,肝、十二指肠、结肠组织抗氧化能力及肠道菌群的影响,评价肉桂精油的摄入对机体健康的潜在影响。方法:将8周龄C57雄性小鼠随机分为3组:对照组和肉桂精油低、高剂量(100、300 mg/(kg·d))组;饲养4周后测定小鼠体质量、肝脏代谢相关酶活性以及肝、十二指肠、结肠组织抗氧化能力,运用Illumina高通量测序技术测定小鼠肠道菌群的变化情况,利用气相色谱-质谱联用仪检测小鼠粪便中短链脂肪酸含量变化情况。结果:肉桂精油的摄入能够显著降低小鼠腹脂指数,使肝脏丙氨酸氨基转移酶活力、肠道抗氧化能力显著提高(P<0.05);摄入肉桂精油后,在门水平上小鼠粪便中软壁菌门(Tenericutes)相对丰度显著降低(P<0.05),在属水平上布劳特氏菌属(Blautia)、真杆菌属([Eubacterium]_fissicatena_group)等条件致病菌相对丰度显著降低(P<0.05),罗氏菌属(Roseburia)、粪芽孢菌属(Coprobacillus)等有益菌相对丰度显著上升(P<0.05);肉桂精油高剂量组小鼠粪便中乙酸、丙酸、总酸的含量显著降低(P<0.05)。结论:肉桂精油的摄入能够调节肠道氧化还原状态平衡和肠道菌群结构,抑制致病菌群生长,促进有益菌群增殖,具有潜在促进机体健康的作用。

Objective:To explore the effect of cinnamon oil on the enzyme activities involved in hepatic metabolism,the antioxidant capacity of liver,duodenum and colon tissues,and the intestinal flora in mice,and to evaluate the potential health benefits of consumption of cinnamon oil.Methods:Eight-week-old male C57 mice were randomly divided into three groups:control,low-dose(100 mg/(kg·d))and high-dose(300 mg/(kg·d))cinnamon oil groups.After 4 weeks of feeding,the body mass,the enzyme activities involved in liver metabolism and the antioxidant capacity of liver,duodenum and colon tissues were measured.Illumina high-throughput sequencing was used to measure the change in gut microbiota at the levels of phylum and genus,and gas chromatography-mass spectrometry was used to detect the change in the contents of short chain fatty acids(SCFAs)in the feces of mice.The results showed that consumption of cinnamon oil significantly reduced the abdominal fat index,and increased the liver alanine aminotransferase activity and the antioxidant capacity of intestinal tissue(P<0.05).At the level of phylum,the relative abundance of Tenericutes in mouse feces was decreased significantly after consumption of cinnamon oil(P<0.05).At the level of genus,the relative abundance of conditional pathogens such as Blautia and[Eubacterium]_fissicatena_group was decreased significantly(P<0.05),while the relative abundance of probiotics such as Roseburia and Coprobacillus was increased significantly(P<0.05).The contents of fecal acetic acid,propionic acid and total acid were decreased significantly in the high-dose cinnamon oil group(P<0.05).These results showed that consumption of cinnamon oil could regulate redox balance and the structure of intestinal flora by inhibiting pathogenic bacteria and promoting the proliferation of beneficial bacteria,thus exerting potential health benefits.