摘要
目的研究长链非编码RNA(lncRNA)前列腺癌相关转录因子1(PCAT-1)在卵巢癌组织中的表达及其对卵巢癌细胞SKOV3增殖、迁移及侵袭能力的影响.方法荧光实时定量PCR(qRT-PCR)检测PCAT-1在卵巢癌组织及癌旁组织中的表达水平.通过小干扰RNA技术抑制SKOV3细胞株PCAT-1的表达,qRT-PCR验证干扰效果,CCK8法检测抑制PCAT-1表达对SKOV3细胞增殖活性的影响,细胞划痕实验、Transwell实验分别检测抑制PCAT-1表达对SKOV3细胞迁移及侵袭能力的影响.Western blot检测抑制PCAT-1后聚腺苷二磷酸核糖聚合酶(PARP1)的蛋白表达变化.结果卵巢癌组织中LncRNA PCAT-1的表达水平显著高于正常癌旁组织.抑制PCAT-1表达后,SKOV3细胞的增殖活性、迁移及侵袭能力减弱,PARP1的蛋白表达量明显下降.结论lncRNA PCAT-1在卵巢癌组织中表达升高,其可能通过调控PARP1参与卵巢癌细胞的增殖、迁移及侵袭过程.
Objective To investigate the expression of long non-coding RNA(IncRNA)PCAT-1 in ovarian cancer tissues and its effect on the proliferation,migration and invasion of ovarian cancer cell line SKOV3.Methods Fluorescence real-time quantitative PCR(qRT-PCR)was used to detect the expression level of PCAT-l in ovarian cancer tissues and adjacent tissues.The expression of PC A T-1 in SKOV3 cells was inhibited by small interfering RNA technology,and the interference effect was verified by qR T-PC R.CCK8 was used to detect the effect ofsi-PC A T-1 on the proliferation activity ot'SKOV3 cells.The wound healing assay and transwell assay were used to detect the effect of si-PCAT-1 on the migration and invasion of SKOV3 cells.Western blot was used to detect the effect of si-PCAT-1 on the protein expression of poly ADP~ribose polymerase(PARIM).Results The expression level of LncRNA PCAT-1 in ovarian cancer rissue was significantly higher than that in normal adjacent tissues.After silencing the expression of PCAT-1,the proliferation activity,migration and invasion ability of SKOV3 cells were inhibited,and the protein expression of PARP1 decreased significantly.Conclusion The expression of ncRNA PCAT-1 is increased in ovarian cancer tissues,and it may participate in the proliferation,migration and invasion of ovarian cancer cells by regulating PARP1.
出处
《浙江临床医学》
2021年第4期471-474,共4页
Zhejiang Clinical Medical Journal
基金
浙江省医药卫生科研面上项目(2018KY822),绍兴市公益性技术应用研究计划项目(2018C30060))。