蜡状芽孢杆菌ZY12产磷脂酶D的影响因素

Factors influencing phospholipase D production by Bacillus cereus ZY12

磷脂酶D是一类酯键水解酶,能够生物转化卵磷脂生成磷酯酰丝氨酸,在食品及医药领域有重要应用。为高效获得磷脂酶D,实现生物转化生产磷酯酰丝氨酸,以前期获得的1株具有磷脂酶D活性的蜡状芽孢杆菌ZY12为发酵菌株,通过单因素试验,确定影响蜡状芽孢杆菌产生磷脂酶D的条件因素。结果表明,20 g/L蛋黄、2 mmol/L MgSO_(4)·7H_(2)O、培养温度30℃、pH为7、培养时间36 h,磷脂酶D活性最高为82 mU/mL。同时研究发现,除蛋黄外,大豆卵磷脂、花生卵磷脂、葵花卵磷脂、水溶性卵磷脂均可诱导菌株ZY12产生磷脂酶D,葡萄糖、果糖、麦芽糖则会抑制磷脂酶D的产生。

Phospholipase D(PLD)is a kind of ester hydrolase,which catalyzes biotransformation of lecithin to phosphatidylserine and therefore shows great potential in food and pharmaceutical industries.In this study,a phospholipase D was produced by Bacillus cereus ZY12,and 82 mU/mL of activity was obtained when the strain was cultured under 30℃and pH 7 for 36 h in medium containing 20 g/L of yolk and 2 mmol/L of MgSO_(4)·7H_(2) O.Further investigation showed that the expression of PLD in this strain could be induced by yolk,lecithin from soybean,lecithin from peanut,and lecithin from sunflower,while was completely inhibited by glucose,fructose and maltose.