CD4+Treg细胞对变应性鼻炎患者外周血单个核细胞的作用

Influences of CD4Tregs on peripheral blood mononuclear cells from allergic rhinitis patients

目的探讨CD4+ Treg细胞对变应性鼻炎(AR)患者外周血单个核细胞(PBMCs)的作用。方法招募正常人(对照组)和AR患者(AR组)各15例,分别从两组志愿者外周血中获取PBMCs,接着从PBMCs中分离、提纯CD4+ Treg细胞,检测该细胞在总CD4+ T细胞中所占的百分比,并将其进行体外培养,然后检测CD4+ Treg细胞培养基中白介素(IL)-10和转化生长因子(TGF)-β及其mRNA的含量,最后,将体外培养的CD4+ Treg细胞加入到AR患者的PBMCs培养基中进行干预,并检测培养基中IL-4和IL-5的浓度。结果 AR患者CD4+Treg细胞(2.523±0.260)在总CD4+ T细胞中所占的百分比较对照组(4.716±0.390)减少,但AR组患者CD4+ Treg细胞培养基中IL-10(96.19±6.96 vs 33.69±3.88)和TGF-β(77.73±7.96 vs 31.39±3.15)及其mRNA(3.30±0.27 vs 0.26±0.05;3.29±0.26 vs 0.26±0.03)的含量却显著增多,AR组PBMCs培养基中IL-4(260.40±12.49)和IL-5(287.10±18.52)的浓度较对照组(23.07±3.26;19.62±2.46)为高,径AR患者的CD4+ Treg细胞干预后,AR患者PBMCs培养基中IL-4(120.60±9.58)和IL-5(137.60±12.33)的浓度显著降低,但对照组CD4+ Treg细胞的干预没有改变培养基中这些细胞因子(241.70±12.44;262.40±18.88)的含量。结论 CD4+ Treg细胞在变应性炎症状态下可以激活并起到抑制炎症的作用。

Objective The present study was aimed to assess the influences of CD4+ Tregs on peripheral blood mononuclear cells(PBMCs) from AR patients.Methods Fifteen normal subjects(normal group) and 15 AR patients(AR group) were enrolled. Their PBMCs were obtained, and CD4+ Tregs were separated and purified from PBMCs with calculation of the percentage of CD4+ Tregs in total CD4+ T cells and then cultured in vitro. The levels of interleukin(IL)-10 and transforming growth factor(TGF)-β and their mRNAs in Tregs cultures were detected. Finally, the in vitro cultured CD4+ Tregs were administered into PBMCs cultures from AR patients and the concentrations of IL-4 and IL-5 in the cultures were measured.Results The percentage of CD4+ Tregs in the total CD4+ T cells from PBMCs of AR group(2.523±0.26) was lower than that of the normal group(4.716±0.39). However, the contents of IL-10(96.19±6.96 vs 33.69±3.88) and TGF-β(77.73±7.96 vs 31.39±3.15) as well as their mRNAs(3.30±0.27 vs 0.26±0.05;3.29±0.26 vs 0.26±0.03) in CD4+ Tregs cultures of the AR group were higher than those of the normal group. The concentrations of IL-4 and IL-5 in PBMCs cultures of the AR group(260.40±12.49;287.10±18.52) were higher than those of the normal group(23.07±3.26;19.62±2.46), and decreased after the administration of CD4+ Tregs from AR patients(120.60±9.58;137.60±12.33). The treatment of CD4+ Tregs from normal subjects did not change concentrations of IL-4(241.70±12.44) and IL-5(262.40±18.88) in PBMCs cultures of the AR patients.Conclusion The CD4+ Tregs may be activated in allergic condition and alleviate inflammatory responses in AR.