侧脑室注射orexin B对MPTP帕金森病模型小鼠运动行为影响

EFFECT OF INTRACEREBROVENTRICULAR INJECTION OF OREXIN B ON MOTOR BEHAVIOR IN A MOUSE MODEL OF PARKINSON’S DISEASE INDUCED BY 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE

目的探究侧脑室注射orexin B对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)帕金森病模型小鼠运动行为的影响。方法将小鼠随机分为正常对照组、MPTP组、orexin B组和orexin B+MPTP组。采用转棒实验观察各组小鼠在旋转棒上停留的时间。结果与正常对照组相比,MPTP组小鼠在旋转棒上停留的时间显著缩短(t=6.83,P<0.01);侧脑室注射orexin B的正常小鼠在旋转棒上停留的时间与正常对照组相比差异无显著性(P>0.05),然而,侧脑室注射orexin B的MPTP模型小鼠在旋转棒上停留的时间较MPTP组明显延长(t=3.17,P<0.05)。结论侧脑室注射orexin B可改善MPTP诱导的小鼠运动障碍。

Objective To investigate the effect of intracerebroventricular injection of orexin B on motor behavior in a mouse model of Parkinson’s disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Mice were randomly divided into normal control group,MPTP group,orexin B group,and orexin B+MPTP group.The rotarod test was used to observe the retention time of mice on the rotarod.Results Compared with the normal control group,the MPTP group had a significant reduction in the retention time on the rotarod(t=6.83,P<0.01).There was no significant difference in retention time on the rotarod between the normal mice with intracerebroventricular injection of orexin B and the mice in the normal control group(P>0.05).Compared with the MPTP group,the orexin B+MPTP group had a significant increase in the retention time on the rotarod(t=3.17,P<0.05).Conclusion Intracerebroventricular injection of orexin B can improve dyskinesia induced by MPTP in mice.