氢水对小鼠闭合性轻型颅脑损伤后焦虑抑郁样行为的改善作用探讨

Ameliorative effect of hydrogen water on mice with anxiety and depression-like behavior after repetitive mild brain injury

目的建立并验证闭合性反复轻型颅脑损伤(rmTBI)动物模型,探讨氢水对rmTBI模型动物的神经保护作用及其导致的行为学结果差异。方法选用C57BL/6小鼠20只,随机分为对照组(4只)、打击组(8只)和干预组(8只),打击组和干预组使用闭合性颅脑打击装置,打击1次/d,连续5 d。对照组和打击组使用纯净水喂养,干预组使用氢水(H2含量0.003‰)喂养,打击后1、3、5、7、15、30 d应用旷场实验、高架十字平台迷宫实验和悬尾实验评价小鼠焦虑抑郁样行为。结果末次打击后3 d,对照组小鼠悬尾实验静止潜伏期显著长于打击组(P<0.05),探索高架十字迷宫开臂时间显著长于打击组(P<0.05),而与干预组均无显著差异(P>0.05);打击后15 d,旷场实验中对照组静止时间显著短于打击组与干预组(P<0.05),攀壁次数显著多于打击组与干预组(P<0.05),而打击组与干预组间差异无统计学意义(P>0.05)。打击后30 d,对照组小鼠高架十字平台迷宫探索开臂时间显著长于打击组及干预组(P<0.05),而打击组与干预组间差异无统计学意义(P>0.05)。结论氢水对rmTBI小鼠伤后早期易激惹、活动减少等症状有一定改善作用,但对预防和治疗伤后中远期的抑郁样行为及学习探索能力障碍无效。

Objective To establish and verify the animal model of repetitive mild traumatic brain injury(rmTBI),and to verify the protective effect of hydrogen water on rmTBI model animals and the behavioral improvement caused by hydrogen water.Methods Twenty C57BL/6 mice were randomly divided into control group(n=4),impact group(n=8)and intervention group(n=8).The impact group and intervention group were treated with closed craniocerebral percussion device once a day for 5 consecutive days.The control group and the impact group were fed with pure water,and the intervention group were fed with hydrogen water(H_(2) content 0.003‰).The open-field test,elevated plus-maze and tail suspension test were used to evaluate the anxiety and depression-like behavior of mice on the 1^(st),3^(rd),5^(th),7^(th),15^(th),30^(th) day-post impact(DPI).Results On DPI 3,the resting latency of the suspended tail test in the control was significantly longer than that in the impact group(P<0.05),whereas it was not significantly different from that in the intervention group;on DPI 3,the open-arm exploring time of elevated plus-mazae by the control group was significantly longer than that of the impact group(P<0.05),whereas it was not significantly different from that of the intervention group;on DPI 15,the resting time of the control group in the open-field test was significantly shorter than that of the intervention group(P<0.05),and the number of wall climbing was significantly greater than that of the intervention group(P<0.05),whereas there was no significant difference between the impact group and the intervention group.On DPI 30,the open arm time of the elevated plus-maze exploration in the control mice was significantly longer than that in the impact and intervention groups(P<0.05),whereas there was no statistical difference between the impact and intervention groups.Conclusion Hydrogen water showed some ameliorative effects on symptoms such as irritability and reduced activity in the early stage after injury in rmTBI mice,but it was not effective in preventing and treating depression-like behaviors and exploratory memory impairment in long term observation after injury.