不同化学药剂处理对休眠期大叶樟种子萌发的影响

Effect of Different Chemical Treatment on Seed Germination of Cinnamomum septentrionale in Dormant Period

为研究大叶樟种子休眠的解除措施,以新采大叶樟种子为材料,研究不同浓度赤霉素(GA3)、过氧化氢(H2O2)、褪黑素(MT)、水杨酸(SA)处理对其萌芽特性和幼苗生长的影响,每种化学药剂设置3个浓度梯度,以蒸馏水处理为对照。结果表明,同一种化学药剂中,2%浓度的H2O2,300 mg·L^(-1)浓度的GA3,200μg·L^(-1)浓度的MT,0.5 mmol·L^(-1)浓度的SA处理能显著提高休眠期大叶樟种子萌发率、萌发势、萌芽指数和萌发后的幼苗质量,浓度过高或过低则效果变差。结合隶属函数法的综合评价值来看,200μg·L^(-1)的MT和300 mg·L^(-1)的GA3处理效果较好,综合隶属函数值分别为0.805,0.761,而500μg·L^(-1)的MT和2 mmol·L^(-1)的SA处理效果较差,综合隶属函数值分别为0.113,0.025。综上,适宜浓度的GA3、MT、H2O2和SA处理均能促进大叶樟种子休眠解除和提高种苗质量,其中以200μg·L^(-1)的MT和300 mg·L^(-1)的GA3处理3 h效果最好。

In order to study the measures to relieve the dormancy of Cinnamomum septentrionale seeds,the effects of different concentrations of GA3,hydrogen peroxide(H2O2),melatonin(MT)and salicylic acid(SA)treatment on the germination characteristics and seedling growth were studied.Three concentration gradients were set up for each chemical agent,and the treatment of distilled water was used as the control.The results showed that in the same chemical agent treatments,the germination rate,germination potential,germination index and seedling quality of Cinnamomum septentrionale seeds in dormant stage were significantly improved by the optimal concentration,such as 2%H2O2,300 mg·L^(-1)GA3,200 g·L^(-1)MT,and 0.5 mmol·L^(-1)SA,while the effect was worse if the concentration was too high or too low.Combined with the results of the membership function method,200μg·L^(-1)MT and 300 mg·L^(-1)GA3 had better treatment effect,with a synthetic membership function value of 0.805 and 0.761 respectively,while 500μg·L^(-1)MT and 2 mmol·L^(-1)SA had worse treatment effect,with a synthetic membership function value of 0.113 and 0.025 respectively.In conclusion,suitable concentrations of GA3,MT,H2O2 and SA could promote the dormancy dissolution and improve the quality of seedlings,which the seeds soaked 3 h with MT of 200 g·L^(-1)and GA3 of 300 mg·L^(-1)had the best effect.