天门冬离体快繁技术

Rapid Propagation in vitro of Asparagus cochinchinensis

以无菌萌发的天门冬(Asparagus cochinchinensis)种子胚轴为外植体,研究植物生长调节物质种类及浓度对愈伤组织诱导、丛生芽分化和植株再生的影响,建立其离体快速繁殖技术。结果表明,愈伤组织诱导的适宜培养基为MS+6-BA 1.0 mg·L^(-1)+NAA 0.5 mg·L^(-1),诱导率95.6%;愈伤组织增殖培养基为MS+6-BA 0.5 mg·L^(-1)+NAA 2.0 mg·L^(-1),增殖倍数为9.7;丛生芽诱导培养基为MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+KT 0.1 mg·L^(-1),诱导率为91.1%;适宜的壮苗培养基为MS+6-BA 0.2 mg·L^(-1)+IAA 1.0 mg·L^(-1);适宜的生根培养基为1/2MS+NAA 2.0 mg·L^(-1),生根率达84.4%。本研究为构建天门冬药材产业化所需种苗生产技术体系奠定了基础。

In order to establish the rapid propagation technique in vitro of Asparagus cochinchinensis,the hypocotyls in seeds by aseptic sprouting were used as explants,the effects of different plant growth regulators on callus induction,shoots differentiation and plants regeneration were studied.The results showed that the suitable medium for callus induction was MS+6-BA 1.0 mg·L^(-1)+NAA 0.5 mg·L^(-1),and the induction rate was 95.6%;The proliferation medium of callus was MS+6-BA 0.5 mg·L^(-1)+NAA 2.0 mg·L^(-1),and the proliferation multiple was 9.7;The induction medium of clustered buds was MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+KT 0.1 mg·L^(-1),the induction rate was 91.1%;The suitable medium for strong seedlings was MS+6-BA 0.2 mg·L^(-1)+IAA 1.0 mg·L^(-1);The suitable rooting medium was 1/2 MS+NAA 2.0 mg·L^(-1),the rooting rate reached 84.4%.This study laid a foundation for the technical system construction of seedlings production in the industrialization of A.cochinchinensis.