马铃薯StCML基因家族鉴定及表达分析

Genome-wide Identification and Expression Analysis of StCML Gene Family in Solanum tuberosum L.

类钙调蛋白(calmodulin-like protein,CML)是植物中一种重要的Ca2+结合蛋白,在植物生长发育和胁迫响应过程中起着重要的作用。该研究通过生物信息学方法在马铃薯基因组中鉴定了StCML基因家族成员,并对它们的表达模式及胁迫响应进行了分析,为深入解析马铃薯StCML基因家族成员在生长发育和胁迫响应中的作用机制奠定理论基础。结果显示:(1)在马铃薯基因组中共鉴定到80个StCML基因,它们均具有EF-hand结构;根据系统进化树拓扑结构可分为5个亚家族,在1~5亚家族中分别含有18、12、14、12、和24个基因,大部分基因具有较为保守的基因结构和基序。(2)RNA-Seq数据分析发现,StCML基因主要在马铃薯的花、叶柄、芽、雄蕊、匍匐茎和块茎中有特异表达,并且主要对盐、热、干旱和赤霉素处理有响应。(3)qRT-PCR分析发现,在低温胁迫下StCML13、StCML21和StCML53表达上调;在高温胁迫下StCML11、StCML21和StCML39表达上调;盐胁迫下StCML21和StCML60表达上调;青枯菌处理下StCML53表达上调,StCML8、StCML 13、StCML 21和StCML 60表达下调。研究表明,StCML基因对多种胁迫均有响应。

Calmodulin-like protein(CML)is a kind of important Ca2+binding protein in plants,which plays a critical role in plant growth,development,and stress response.In this study,StCML gene family members were identified in potato genome by bioinformatics methods,and their expression patterns and stress response were analyzed,which laid a theoretical foundation for further understanding the mechanism of StCML gene family members in growth and development and stress response.The results showed that:(1)a total of 80 StCML genes were identified from the Solanum tuberosum L.genome,and all of them have the EF-hand domains.According to the topological structure of phylogenetic tree,they were divided into 5 subfamilies,each of which has respectively 18,12,14,12,and 24 members,and most of the members have relatively conservative gene structures and motifs;(2)Through the analysis of RNA-Seq data,it was found that StCML genes showed tissue-specific expression in flowers,petioles,shoots,stamens,stolons and tubers,they were mainly responded to salt,heat,drought stress and GA3.(3)qRT-PCR analysis showed StCML13,StCML21 and StCML53 were up-regulated under cold stress,StCML11,StCML21 and StCML39 were up-regulated under heat stress,StCML21 and StCML60 were up-regulated under salt stress,StCML53 was up-regulated under Ralstonia solanacearum infection,indicating that StCML family is able to response to multiple stresses.These results could serve as the foundation for in-depth research on the mechanism of the StCML gene family regulating plant stress tolerance.